Endophytic fungus for producing triptolide

A technology of triptolide and endophytic fungi, applied in fungi, microorganisms, microorganism-based methods, etc., can solve the problems of low component content, small reserves of wild resources, long harvest period, etc.

Inactive Publication Date: 2012-01-18
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the content of medicinal components of Tripterygium wilfordii is extremely low, coupled with the small reserves of wild resources, and artificial cultivation has the problems of slow growth and long harvest period, which is far from meeting

Method used

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  • Endophytic fungus for producing triptolide
  • Endophytic fungus for producing triptolide
  • Endophytic fungus for producing triptolide

Examples

Experimental program
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Embodiment Construction

[0011] The isolation and screening of bacterial strain NS-12 are as follows:

[0012] (1) Preparation of Tripterygium wisteria plant tissue: The strain is mainly obtained from different tissues and organs of the wild Tripterygium wisteria plant, and the plants are collected by dividing the plant into main roots, lateral roots, main branches, side branches, leaves and petioles.

[0013] (2) Surface disinfection of plant materials: soak leaves in 70% alcohol for 40s, 0.1% mercury liter for 90s, branches and stems in 70% alcohol for 30s, 0.1% mercury liter for 60s, root disinfection in 70% alcohol for 50s , Soak in 0.1% mercury liter for 120s;

[0014] (3) Surface sterility test of Tripterygium wilfordii tissue: the collected Tripterygium wilfordii tissue was surface-sterilized, planted directly on solid medium, placed in an incubator for 72 hours, and the tissue without any bacterial growth on the surface was selected. . This process uses PDA solid medium, the formula is fresh...

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Abstract

The invention provides an endophytic fungus for producing triptolide. The endophytic fungus is a Penicillium steckii strain NS-12 and is collected in China General Microbiological Culture Collection Center, and the collection number is CGMCC No. 4928. Fermentation culture conditions for producing the triptolide by using the endophytic fungus are that: a potato dextrose agar (PDA) liquid culture medium (the liquid loading capacity is 100ml/500ml) is used, the shake culture is performed by a shaker at the rotating speed of 150r/min, the culture temperature is 28 DEG C, and the culture time is 72 to 144h. Through suspension culture, the strain can secrete a considerable amount of triptolide out of cells in a short time; the method is remarkably superior to an acquisition way of the traditional plant cultivation; and the strain has good industrialized application prospect.

Description

technical field [0001] The invention relates to an endophytic fungus producing triptolide. Background technique [0002] Since Vogl isolated the first strain of endophytic fungi from ryegrass seeds in 1898, plant endophytes have received extensive attention as a new microbial resource, and finding and discovering new active compounds from endophytes has become a Another hot spot of research at home and abroad. Studies have shown that endophytic fungi exist in all plant species, and endophytic fungi in medicinal plants can produce the same or similar medicinal active ingredients as host plants, especially many new active ingredients have been found, which have been proven to have Obvious anticancer, antibacterial, antioxidant and other activities. With the continuous deepening of research on medicinal plant resources, some endophytic fungi parasitic in medicinal plants have been found to not only significantly promote the production of host secondary metabolites, but also p...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/80
Inventor 封磊宋萍洪伟吴承祯朱留刚
Owner FUJIAN AGRI & FORESTRY UNIV
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