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Method for separating adipose-derived mesenchymal stem cells from tumescent liquid

A technique for adipose stem cells and mesenchymal stem cells, applied in the field of isolating adipose mesenchymal stem cells

Inactive Publication Date: 2012-01-25
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No one has reported a patent on the separation of adipose stem cells from swelling fluid

Method used

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  • Method for separating adipose-derived mesenchymal stem cells from tumescent liquid
  • Method for separating adipose-derived mesenchymal stem cells from tumescent liquid
  • Method for separating adipose-derived mesenchymal stem cells from tumescent liquid

Examples

Experimental program
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Embodiment

[0039] 1. Obtaining expansion fluid

[0040] Before liposuction, infuse excess hypotonic swelling fluid into the site to be extracted, and then put about 500ml of the mixture of fat and swelling fluid obtained during liposuction at room temperature for 10 minutes. After the fat is completely separated from the swelling fluid, pipette extraction The lower red liquid is the swelling liquid containing fat stem cells.

[0041] 2. Treatment of expansion fluid

[0042] Put the collected expansion fluid into a centrifuge and centrifuge at 1500 rpm for 5 min. Centrifuge to aspirate and discard the liquid on the upper part of the centrifuge tube, and collect the precipitate. Suspend each tube with 3ml PBS, add red blood cell lysate in a volume ratio of 1:2, let it stand at room temperature for 5 minutes, centrifuge at 1200rpm for 3min, discard the supernatant and take the pellet for later use.

[0043] 3. Isolation of adipose stem cells

[0044] The obtained precipitate was suspended in low-s...

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Abstract

The invention discloses a method for separating adipose-derived mesenchymal stem cells from tumescent liquid in the surgical liposuction surgery, and provides a new adipose-derived stem cell. The new adipose-derived stem cell does not have obvious difference with the adipose-tissue-derived mesenchymal stem cell in the aspects of cell morphology, immunophenotype and the like. Thus, a new way is provided for separating the adipose-derived stem cell.

Description

Technical field [0001] The invention relates to a method for separating adipose mesenchymal stem cells from a swelling liquid, and belongs to the field of biomedicine. Background technique [0002] Mesenchymal stem cells [mesenchymal stem cells, MSC] are important members of the stem cell family, derived from the early mesoderm and ectoderm. MSC was first discovered in bone marrow, and has attracted increasing attention because of its multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. For example, mesenchymal stem cells can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, myocardium, endothelium and other tissue cells under specific induction conditions in vivo or in vitro. After continuous subculture and cryopreservation It still has the potential for multidirectional differentiation, and can be used as an ideal seed cell to repair tissue and organ da...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 郑榆坤海泉
Owner CHENGDU QINGKE BIOTECH