Detection method for structural change of rhizosphere soil microorganism of genetically modified paddy rice
A technology for transgenic rice and rhizosphere soil, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as restriction of transgenic soybean varieties, and achieve the effect of rich map bands, accurate measurement, and overcoming interference.
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[0017] rice material
[0018] Bt rice Huachi B6, parent Jiazao 935 and distant parent Zhongjiu B
[0019] Field Trials and Sampling
[0020] The field experiment was carried out in the seedling base of Jiande City, Zhejiang Province. The experimental field was divided into 9 plots, and the three rice varieties were planted respectively. Each variety had 3 repeated plots, and each plot had 26-28 rows×12 plants / row≥ There are 300 plants, and the plots of different varieties are set at intervals.
[0021] Sampling was performed at the tillering, heading, filling and maturity stages of rice. The rice and the soil at its roots were pulled out with a spatula and brought back to the laboratory, and three rice samples were taken from each plot. After the retrieved samples were moderately air-dried at room temperature, the soil around the rhizosphere was shaken off and collected. After removing debris such as plant roots and gravel, they were passed through a 2mm sieve for later use...
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