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Culture method for liquid fermentation of rare edible-medicinal fungus Sparassis crispa and special medium thereof

A liquid fermentation and liquid culture technology, applied in the directions of botanical equipment and methods, applications, fungi, etc., can solve problems such as strain instability, and achieve the effects of good social and economic benefits, low cost, and simple raw materials

Active Publication Date: 2013-07-10
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Literature survey finds that the research of hydrangea cultivation is mostly solid culture, although there are a few reports of liquid fermentation, You Xiong et al. 41-45) and Liu Chengrong (Research on liquid fermentation conditions of Hydrangea mutant strains, Jiangxi Agricultural University Journal 2008 (5): 898-902) both used mutant strains of Hydrangea to study, and mutant strains are used in health food and medicine. its limitations, and the strain itself is unstable

Method used

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  • Culture method for liquid fermentation of rare edible-medicinal fungus Sparassis crispa and special medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the production of liquid fermentation hydrangea mycelium

[0022] The formula of the special liquid fermentation medium for Hydrangea: 200 grams of potatoes, 40.0 grams of brown sugar, 3.0 grams of peptone, 3.0 grams of soybean oil, KH 2 PO 4 3.0g, MgSO 4 1.5g, 100ml of pine needle juice, dilute to 1000mL with water, adjust pH to 4.0.

[0023] Control medium: 200 grams of potatoes, 20.0 grams of glucose, natural pH, dilute to 1000 ml with water.

[0024] 1. The preparation of culture medium

[0025] Potato processing: Peel the potatoes, cut into small pieces and boil for half an hour, then filter through gauze to get potato juice.

[0026] Preparation of pine needle juice: collect the fallen pine needles, take 20 grams of pine needles, add 100ml of water to boil for half an hour, filter to 100ml.

[0027] Add pine needle juice, brown sugar, peptone, soybean oil, KH to potato juice according to the above formula 2 PO 4 , MgSO 4 Dissolve, and final...

Embodiment 2

[0035] Embodiment 2, the production of liquid fermentation hydrangea mycelium

[0036] Hydrangea special liquid fermentation medium: 50.0 grams of corn flour, 20.0 grams of glucose, 3.0 grams of peptone, 3.0 grams of soybean oil, KH 2 PO4 2.0g, MgSO 4 1.0g, 50ml of pine needle juice, dilute to 1000ml with water, adjust pH to 3.0.

[0037] Control medium: 200 grams of potatoes, 20.0 grams of glucose, dilute to 1000 ml with water, and the pH value is natural.

[0038] Preparation of medium: boil corn flour for half an hour, then filter with gauze; add pine needle juice, glucose, peptone, KH according to the above formula 2 PO 4 , MgSO 4 and soybean oil, and finally the volume was adjusted to 1000 ml with water, and the pH value was adjusted to 3.0.

[0039] Hydrangea strains (preservation number: ACCC 51488, purchased from China Agricultural Microorganism Strain Resource Bank) were inoculated on potato solid medium (PDA) for activation at a culture temperature of 25-28° C...

Embodiment 3

[0042] Embodiment 3, the production of liquid fermentation hydrangea mycelium

[0043] Hydrangea special liquid fermentation medium: 20.0 grams of soluble starch, 80 ml of pine needle juice, 2.5 grams of peptone, 2.0 grams of soybean oil, KH 2 PO 4 5.0g, MgSO 4 2.5g, dilute to 1000ml with water, and adjust the pH value to 5.0.

[0044] Control medium: 200 grams of potatoes, 20.0 grams of glucose, dilute to 1000 ml with water, and the pH value is natural.

[0045] Preparation of culture medium: first mix the soluble starch thoroughly with cold water, add boiling water to fully dissolve, add peptone, pine needle juice, KH 2 PO 4 , MgSO 4 and soybean oil, and finally set the volume to 1000 ml with water, and adjust the pH value to 5.0.

[0046] Hydrangea strains (preservation number: ACCC 51488, purchased from China Agricultural Microorganism Strain Resource Bank) were inoculated on potato solid medium (PDA) for activation at a culture temperature of 25-28° C. for 14 days...

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Abstract

The invention discloses a special medium for the liquid fermentation of a rare edible-medicinal fungus Sparassis crispa and a culture method thereof. 1ml of the medium contains 20.0-200.0g of a composite carbon source, 0-60.0g of sugar, 0-5.0g of peptone, 0-5.0g of KH2PO4, 0-2.5g of MgSO4, 0-10.0ml of a pine needle juice, 0-3.0g of plant oil, and the balance water, and the pH value of the medium is adjusted to 2.0-6.0. The culture method comprises the following steps: 1, inoculating strains of Sparassis crispa to the special medium for the liquid fermentation of Sparassis crispa according to an inoculation amount of 5-20%, wherein the initial pH value of the medium is 2.0-6.0; and 2, carrying out oscillation culture under natural illumination conditions, wherein the temperature is 20-30DEG C, and the rotation speed is 100-200rpm. The method of the invention breaks through present limitations of solid culture and artificial cultivation of Sparassis crispa; and the fermentation production of the Sparassis crispa, which is carried out by using the medium and the culture method of the present invention, has the advantages of fast growth speed, short period, high yield, simple process,low cost and the like, can be adapted to the industrialized fermentation production of Sparassis crispa.

Description

technical field [0001] The invention relates to a liquid fermentation culture method of a rare edible and medicinal fungus hydrangea and a special culture medium thereof. Background technique [0002] Hydrangea [Sparassis crispa (Wulf.) Fr] is a rare edible and medicinal fungus, belonging to Kingdom Fungi (Kingdom Fungi), Basidiomycota (Basidiomycota), Polyporales (Polyporales), Hydrangeaceae in biological classification (Sparassidaceae), Hydrangea (Sparassis). Hydrangea is distributed in Jilin, Heilongjiang, Yunnan and other places in my country, and also in Japan, Europe, and North America. It is born in spruce, fir or pine forests and mixed forests in late summer and early autumn. [0003] The main active ingredient of hydrangea is rich in β-glucan, which is 43.6% as determined by the Japan Food Analysis Laboratory, which is more than twice that of Ganoderma lucidum and Agaricus blazei, the most among mushrooms, and has high medicinal and health benefits. It has the tit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G3/00A01G1/04
Inventor 董彩虹
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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