Method utilizing magnetic particles to purify deoxyribonucleic acid (DNA) from samples containing trace nucleic acid
A technology in magnetic particles and samples, applied in DNA preparation, recombinant DNA technology, etc., can solve the problems of not involving purification results, prolonging experimental operation time, and complicated steps, shortening the time for DNA extraction and reducing physical shearing. The effect of cutting and easy operation steps
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0049] Example 1: The method and effect verification of purifying genomic DNA from blood spots:
[0050] 1. Purification of DNA
[0051] (1) Sample cracking: Utilize a hole puncher to cut the 2 Put the blood spot into a 1.5ml centrifuge tube, add 150μl guanidinium salt lysate successively (the concentration of guanidine salt lysate is 6M, the composition is: 3~6M GuHCl, 3~6M GuSCN, 0.005~0.01M EDTA, 0.005~ 0.01M NaCl, 4-6% Triton X-100 (volume percentage) and 15-80% (volume percentage) alcohol.) and 1.5 μl of DTT (1M), vortex shaker, 95 ° C water bath Fully lyse for 30min.
[0052] (2) Pick out the fully lysed blood spot sample from the carrier.
[0053] (3) Nucleic acid is adsorbed on the solid phase carrier
[0054] Add 10 μl of magnetic particles with a concentration of 30 mg / ml to the lysate obtained in step (2), mix by vortexing, and let stand for 5 minutes to form a magnetic particle-DNA complex.
[0055] (4) Cleaning: take out the centrifuge tube from the magnetic ...
Embodiment 2
[0063] Example 2: The method and effect verification of purifying genomic DNA from oral swabs:
[0064] Oral swab preparation, rinse your mouth with water three times, gently wipe both sides of the mouth with a swab swab, dry in the shade, and set aside.
[0065] 1. Purification of DNA
[0066] When purifying, cut out a thin layer around the oral cavity cotton swab containing oral mucosal cells as an experimental sample. Subsequent steps are the same as in Example 1.
[0067] 2. STR multiplex amplification analysis
[0068] The DNA obtained after purification is carried out to STR composite amplification analysis, and the STR composite amplification analysis method of embodiment 1 is identical, and the result is as follows image 3 As shown, the STR locus is complete, the typing is accurate, and there is no allele loss, unbalanced amplification, etc., indicating that the purification method has high purification efficiency, and the obtained DNA has high purity and good inte...
Embodiment 3
[0069] Embodiment 3: the method and effect verification of purifying genomic DNA from cigarette butt:
[0070] 1. Sample preparation:
[0071] Generally speaking, for cigarette butts with obvious bite marks, we usually cut the part above the bite mark, and for cigarette butts without obvious bite marks, we try to cut the upper end of the cigarette butt, generally cutting a piece with a size of about 2cm×2cm. Shred it as much as possible.
[0072] 2. Purification of DNA
[0073] Step is identical with embodiment 1.
[0074] 3. STR multiplex amplification analysis
[0075] The DNA obtained after purification is carried out to STR composite amplification analysis, and the STR composite amplification analysis method of embodiment 1 is identical, and the result is as follows Figure 4 As shown, the STR locus is complete, the typing is accurate, and there is no allele loss, unbalanced amplification, etc., indicating that the purification method has high purification efficiency, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com