Method for preparing bacterial cellulose by using decayed fruits as raw materials

A technology for bacterial cellulose and fruit, applied in the field of bacterial cellulose preparation, can solve the problems of high raw material cost, low yield and high cost, and achieve the effects of simple preparation method, low cost and increased bacterial cellulose yield

Inactive Publication Date: 2012-03-28
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are still some problems in the large-scale production of bacterial cellulose, such as high cost of raw materials, low output, etc.
At present, the fermentation and synth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] After the rotten apples are pressed, the juice is collected. Pure water is used to prepare fruit juice with a total sugar content of 2.5 wt%. The juice liquid was sterilized by using a high-pressure steam sterilizer, then inserted into Acetobacter xylinum, and cultured at 30° C. for 5 days to obtain a bacterial cellulose film.

Embodiment 2

[0024] After the rotten peaches are pressed, the juice is collected. Pure water is used to prepare fruit juice with a total sugar content of 5 wt%. The fruit juice liquid was sterilized by using a filter membrane with a pore size of 0.22 microns, then inserted into Acetobacter xylinum, and cultured statically at 30° C. for 6 days to obtain a bacterial cellulose membrane.

Embodiment 3

[0026] Squeeze rotten watermelons to collect the juice. Pure water is used to prepare fruit juice with a total sugar content of 3 wt%, and 0.1 wt% of yeast extract and 0.1 wt% of tryptone are added at the same time. The juice liquid was sterilized by using a high-pressure steam sterilizer, then inserted into Acetobacter xylinum, and cultured at 30° C. for 6 days to obtain a bacterial cellulose film.

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PUM

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Abstract

The invention relates to a method for preparing bacterial cellulose by using decayed fruits as raw materials, which comprises the following steps: 1) squeezing the decayed fruits and collecting juice, preparing juice with 2.5-5wt% of total sugar content by pure water and then disinfecting, carrying out membrane filtration and degerming to obtain the treated juice; 2) putting bacterial cellulose in the treated juice to prepare bacterial strain, standing under the temperature of 20-30 DEG C and culturing, or dynamically culturing under the temperature of 20-30 DEG C and the rotating speed of 50-400rpm, culturing for 5-20 days to obtain the bacterial cellulose. The invention has the advantages of simple preparation method, cheap and easy raw material acquisition and low cost, and is suitable for large scale production; the prepared bacterial cellulose has advantages of high output and wide application prospect.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose preparation, in particular to a method for preparing bacterial cellulose by using rotten fruit as a raw material. Background technique [0002] Bacterial Cellulose (BC) is a natural polymer produced by microbial fermentation. It has the same chemical composition as plant cellulose, but exhibits unique physicochemical and mechanical properties, including high crystallinity, high water holding capacity, ultrafine nanofiber network, high tensile strength and elastic modulus, etc. It is used in many fields such as food, papermaking, medical materials, and sound diaphragms. It is a new type of material that has attracted much attention at home and abroad. [0003] At present, there are still some problems in the large-scale production of bacterial cellulose, such as high cost of raw materials and low yield. At present, the fermentation and synthesis of bacterial cellulose mainly uses carbohydrates ...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065C12R1/01
Inventor 杨光王彩霞
Owner DONGHUA UNIV
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