Kit for extracting nucleic acid by magnetic bead-microtiter plate method and method for extracting nucleic acid by same

A technology for extracting nucleic acid and microwell plate, which is applied in DNA preparation, recombinant DNA technology, etc., which can solve the problems of inconvenient opening and closing repeatedly, and achieve the effect of cheap consumables, less consumption of reagents and economical consumables

Inactive Publication Date: 2012-04-18
南宁海关技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing magnetic bead method for extracting nucleic acid also has the following disadvantages: 1. Since the extraction is performed in a centrifuge tube, it is necessary to repeatedly open the cap to add liquid, mix the cap, and then open the cap to discard the liquid. It is very inconvenient to open the cover repeatedly when the sample is 100%
2. The amount of samples used for molecular biology testing is generally 1-5 microliters, while the conventional one-time extraction volume is 50-100 microliters. Compared with the expensive cost of magnetic beads, there is undoubtedly a large waste

Method used

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  • Kit for extracting nucleic acid by magnetic bead-microtiter plate method and method for extracting nucleic acid by same
  • Kit for extracting nucleic acid by magnetic bead-microtiter plate method and method for extracting nucleic acid by same
  • Kit for extracting nucleic acid by magnetic bead-microtiter plate method and method for extracting nucleic acid by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] As shown in the accompanying drawing, use polystyrene to make a microwell 4 with a volume of 400 microliters and a U-shaped 8-hole at the bottom, and then put the microwell 4 into the corresponding hole frame 3 to form a detachable microwell Plate 2; tap the assembled detachable microplate 2 on the magnetic plate 1 whose shape and size match the bottom of the detachable microplate 2, so that the bottom of the microwell 4 is closely attached to the magnetic plate 1. Get the magnetic bead-microwell plate method extraction nucleic acid kit.

Embodiment 2

[0035] As shown in the attached figure, a microwell 4 with a volume of 500 microliters and a U-shaped bottom of the hole is made of polypropylene, and the microwell 4 is put into the corresponding hole frame 3 to form a detachable microwell plate. 2. Put the assembled detachable microplate 2 on the magnetic plate 1 whose shape and size match the bottom of the detachable microplate 2, so that the bottom of the microwell 4 is closely attached to the magnetic plate 1, and you can get Magnetic bead-microplate nucleic acid extraction kit.

Embodiment 3

[0037] A single-hole microwell 4 with a volume of 550 microliters and a V-shaped bottom of the hole is made of polyethylene, and then a plurality of single-hole microwells 4 are put into a corresponding number of hole frames 3 to form a detachable microwell plate 2; The assembled detachable microplate 2 is tapped on the magnetic plate 1 whose shape and size match the bottom of the detachable microplate 2, so that the bottom of the microwell 4 is closely attached to the magnetic plate 1, and the magnetic beads can be obtained- Kit for nucleic acid extraction by microplate method.

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Abstract

The invention relates to a kit for extracting nucleic acid by a magnetic bead-microtiter plate method and a method for extracting nucleic acid by the same. The kit comprises a kit body and a reagent, wherein, the kit body consists of a detachable microtiter plate (2) similar to an Elisa plate as well as a magnetic plate (1), and the reagent comprises magnetic beads, lysis-binding solution, washing solution A, washing solution B and eluent; the detachable microtiter plate (2) consists of micropores (4) and a pore frame (3), the micropores (4) are made from polystyrene, polyethylene or polypropylene, the bottom of each micropore is in a 'U' or 'V' shape, and the micropores (4) are classified into two forms, namely single pores and multi-connected pores; and the magnetic plate (1) is matched with the bottom of the detachable microtiter plate (2) in shape and size. The kit can be used for extracting a large quantity of the nucleic acid from a sample at one time, and has the advantages of low cost and high operating efficiency, and is simple in operation.

Description

technical field [0001] The invention relates to a kit for extracting nucleic acid and a method for extracting nucleic acid, in particular to a kit for extracting nucleic acid by a magnetic bead-microwell plate method and a method for extracting nucleic acid. Background technique [0002] Nucleic acid is a kind of bioinformatics macromolecular compound. It has been more than 100 years since it was first isolated from cells. It was not until the middle of the last century that scientists made great progress in the structure and function of nucleic acid. Nucleic acid is divided into deoxyribonucleic acid and ribonucleic acid, and nucleotide is the basic unit of nucleic acid. Both DNA and RNA exist in plant and animal species, but a virus contains only one type of nucleic acid, DNA or RNA. The irreplaceable role of nucleic acid in the whole molecular biology has been the main object of molecular biology research since the founding of molecular biology. Therefore, the extractio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 盘宝进温和心谢永平
Owner 南宁海关技术中心
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