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Tree peony root bark extract, as well as preparation method and application thereof

A technique for extracting and using Cortex Moutan, applied in the field of traditional Chinese medicine, can solve problems such as undiscovered, affecting virus secretion, etc.

Active Publication Date: 2012-04-25
云南白药中草药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there is no report on the effect of Moutan cortex extract on GRP78 to affect virus secretion

Method used

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  • Tree peony root bark extract, as well as preparation method and application thereof
  • Tree peony root bark extract, as well as preparation method and application thereof
  • Tree peony root bark extract, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Preparation of Cortex Moutan extract using 50% ethanol-water solution

[0046] The preparation method of the Cortex Moutan extract of Example 1 is as follows figure 1 The flowchart is shown. Specifically, 10 kg of dried root bark of Paeonia suffruticosa Andr. of the Ranunculaceae family Paeonia suffruticosa Andr. was added to 100 kg of 50% ethanol-water solution at room temperature, stirred and soaked for 8 hours, after which the extract was filtered out, and the filter residue was added to 80 kg 50% at room temperature Stir and soak in ethanol-water solution for 8 hours, filter out the extract, add the filter residue to 60kg of 50% ethanol-water solution at room temperature, stir and soak for 8 hours, combine the three extracts and concentrate under reduced pressure to 1 / 30 volume. Press drying or vacuum freeze-drying to obtain the tree peony bark extract.

Embodiment 2

[0047] Example 2. Preparation of Paeonia Suffruticosa Bark Extracts Using Ethanol Solutions of Different Concentrations (30%, 40%, 50%, 60%, 70%)

[0048] reference figure 1 As shown in the flow chart, the Cortex Moutan extract was prepared. Specifically, 5 parts of Paeonia Suffruticosa Bark (each 1 kg) were added to a corresponding concentration (30%, 40%, 50%, 60% or 70%) of ethanol aqueous solution (10 kg) at room temperature and soaked for 8 hours under stirring. After filtering, the first filtrate and the first filter residue are separated. The first filter residue was added to an ethanol aqueous solution (8 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and soaked for 8 hours under stirring. After filtering, the second filtrate and the second filter residue are separated. The second filter residue was added to an ethanol aqueous solution (6 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and soaked fo...

Embodiment 3

[0049] Example 3. Screening of active ingredients using herbal chips

[0050] Using the high-throughput screening method for active ingredients in herbal medicines described in Chinese Patent ZL01121114.8, the extract of Paeonia Suffruticosa Bark obtained in Example 2 was screened. The specific description is as follows (the screening results are shown in Figure 4 ):

[0051] ①Classification of herbal extracts by HPLC

[0052] In Example 2, five different concentrations of ethanol aqueous solution were used to extract the Paeonia suffruticosa bark extract. An appropriate amount of the obtained freeze-dried powder was taken and dissolved in a 50% ethanol aqueous solution (1ml) to obtain the equivalent of 1g crude drug / ml The solution. The obtained solution was centrifuged at 12000 rpm (Shanghai Anting TGL-16B) for 20 minutes, and the clear supernatant was collected. The clarified supernatant was diluted 2 times with 50% ethanol-water solution and filtered with a 0.45 μm syringe fi...

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Abstract

The invention provides a tree peony root bark, as well as a preparation method and application thereof. The preparation method comprises the following steps: extracting a tree peony root bark medicinal material with organic alcohol aqueous solution, concentrating and drying to obtain the tree peony root bark extract. The tree peony root bark extract can inhibit glucose-regulated protein GRP78, and is used for resisting virus. In-vitro experiments prove that: the tree peony root bark extract has remarkable effect in resisting hepatitis B virus secretion, has small toxicity to normal cells and liver cancer cells, and has excellent application prospect.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine, and specifically relates to a traditional Chinese medicine extract and a preparation method and application thereof, and in particular to a tree peony bark extract and a preparation method thereof. The extract can be used for antivirus. Background technique [0002] Paeonia suffruticosa, also known as peony cortex and pink peony, is the dried root bark of Paeonia suffruticosa Andr. First published in "Shen Nong's Materia Medica", now included in "Chinese Pharmacopoeia", slightly cold in nature, bitter, pungent, home to heart, liver and kidney. It has the effects of clearing away heat, detoxifying, promoting blood circulation and removing blood stasis. It is used for the treatment of spots caused by warm toxins, vomiting blood and bleeding, night heat and early cooling, no sweating bone steaming, amenorrhea, dysmenorrhea, carbuncle, sore, bruises, etc. [0003] The main chemical components o...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P31/14A61P31/20A61P31/22A61K125/00
CPCY02A50/30
Inventor 杨晓源黎耀基张大兹黄敏
Owner 云南白药中草药科技有限公司
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