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Method for prolonging telomeres of pluripotent stem cells

A technology of adult stem cells and pluripotent stem cells, which is applied in the field of extending adult pluripotent stem cell telomeres, can solve problems affecting encoded proteins, etc., and achieve the effect of simple operation and low cost

Inactive Publication Date: 2012-05-02
黄必录
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Problems solved by technology

[0021] ATRX is a chromatin reorganization adenosine triphosphate enzyme in the SNF-2 family. It has been intensively studied in a thalassemia-mental retardation X-linked syndrome. More than 100 mutations of ATRX have been found to affect the coding protein, but still remain partially active

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Embodiment Construction

[0035] The present invention is described in further detail below.

[0036] A method of extending telomeres in adult pluripotent stem cells, characterized by:

[0037] Solution one step:

[0038] 1. Introduce the antibody of ATRX or DAXX protein, or the asONs or siRNA of ATRX or DAXX gene into the cultured pluripotent adult stem cells by means of electroporation, liposome or microinjection;

[0039] 2. Then culture the pluripotent adult stem cells in the culture medium for a period of time, and the culture time can be determined according to the required telomere length, generally 3 days to a week, so that the telomeres can be extended;

[0040] 3. The antibody or asONs should be added every 2 hours, because the antibody or antisense oligonucleotide will be degraded by the cells, that is, there is a half-life.

[0041] 4. It is also possible to introduce hTRT into pluripotent adult stem cells at the same time to increase the elongation efficiency of telomeres;

[0042] Solu...

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Abstract

The invention belongs to the field of biomedicine and particularly relates to a method for telomeres of pluripotent stem cells. Individual aging is result from shrinkage of telomeres of pluripotent stem cells; therefore, as long as the telomeres of pluripotent stem cells are prolonged into the save level as the telomeres of pluripotent stem cells of young people, the functions of aged organs in an individual can be revered to the level of the young people. By selectively and temporarily inhibiting the expression of one gene in apoptoxin (ATPX) or death domain-associated protein (DAXX) and the activity of protein instead of inhibiting two genes at the same time, the telomeres of various stem cells can be induced to undergo homologous recombination to be prolonged; so the operation is simplified and the cost is saved.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a method for extending the telomeres of adult pluripotent stem cells. Background technique [0002] Pluripotent adult stem cells are a type of stem cells that can self-renew and differentiate into various tissue cells in individuals, such as hematopoietic stem cells and Muse cells. [0003] 1. The individual is composed of stem cells and non-stem cells [0004] Organs and tissues in individuals are composed of stem cells and non-stem cells. Non-stem cells are a type of functional cells in organs and tissues. They usually cannot renew themselves. When they are reduced due to telomere shortening, DNA mutation and other reasons, they can be differentiated by stem cells. Supplement; the death of stem cells due to cell differentiation, DNA mutation and other reasons can also be supplemented by increasing the self-renewal rate. [0005] All somatic cells including brain neurons, an average...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12N5/0775C12N5/0789C12N5/10C12N15/87
Inventor 黄必录
Owner 黄必录
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