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Micro-molecular interference ribonucleic acid (RNA) for inhibiting glioma proliferation and promoting glioma apoptosis as well as preparation method and application thereof

A small molecule interference, glioma technology, applied in the biological field, can solve the problems that have not yet been seen, and achieve the effect of increasing cell apoptosis

Active Publication Date: 2012-05-02
上海生博生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no report about the small interfering RNA of the STAT3 gene used to inhibit the proliferation of glioma and promote its apoptosis

Method used

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  • Micro-molecular interference ribonucleic acid (RNA) for inhibiting glioma proliferation and promoting glioma apoptosis as well as preparation method and application thereof
  • Micro-molecular interference ribonucleic acid (RNA) for inhibiting glioma proliferation and promoting glioma apoptosis as well as preparation method and application thereof
  • Micro-molecular interference ribonucleic acid (RNA) for inhibiting glioma proliferation and promoting glioma apoptosis as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Glioma cell culture:

[0028] Glioma cell line U251 (purchased from Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) was cultured at 37°C, 5% CO 2 Constant temperature incubator. The cells in the logarithmic growth phase were digested with trypsin to remove the wall, then added an appropriate amount of culture medium and repeatedly pipetted to mix the cells; transfer the obtained cell suspension into a 15ml centrifuge tube, and centrifuge at 1500rpm for 3min; remove the supernatant; use 5ml sterile 1×PBS to suspend the cells, pipette and mix well; add about 1ml of cell suspension to a new culture bottle with 5ml of fresh medium, put it in 37℃, 5%CO 2 Cultivate in an incubator, and passage once in about 2 days (mainly check whether the cells are confluent in the culture flask).

[0029] 2. Isolation of human glioma cells:

[0030] Take human glioma surgical specimens, immerse them in KRBB solution (common Krebs-Ringer ...

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Abstract

The invention discloses micro-molecular interference ribonucleic acid (RNA) for inhibiting glioma proliferation and promoting glioma apoptosis as well as a preparation method and application thereof. The micro-molecular interference RNA has base sequences shown as SEQ ID NO.1 and SEQ ID NO.2. According to the invention, the micro-molecular interference RNA of a gene which has effects on inhibiting the glioma proliferation and promoting the glioma apoptosis is designed, a glioma cell model is utilized to research the effects of the micro-molecular interference RNA on inhibiting the glioma proliferation and the promoting glioma apoptosis, and a new method is provided for gene therapy of glioma.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a small interfering RNA (i.e. shRNA), in particular to a small interfering RNA for inhibiting glioma proliferation and promoting its apoptosis; in addition, the present invention also relates to the small interfering RNA (shRNA). Preparation method and application of molecular interference RNA. Background technique [0002] As an important member of the signal transducer and activator of transcription family (STATs), STAT3 protein plays an important regulatory role in cell survival, invasion, apoptosis and angiogenesis, and is closely related to a variety of malignant tumors. [0003] Glioma is a tumor that occurs in the neuroectoderm, so it is also called neuroectodermal tumor or neuroepithelial tumor. Tumors originate from mesenchymal cells, namely glia, ependyma, choroid plexus epithelium, and nerve parenchymal cells, namely neurons. Most tumors originate from different ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/10A61K48/00A61P35/00
Inventor 郑大赵俊玲夏清梅潘讴东
Owner 上海生博生物医药科技有限公司
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