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Method for detecting illegal cooking oil by using liquid chromatography-mass spectrometry (LC-MS) instrument

A technology of LC-MS and waste oil, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of difficult qualitative and quantitative determination, complex composition of waste oil, and rare detection of waste oil, etc.

Active Publication Date: 2013-05-08
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few research reports on the detection of waste oil. The main reason is that the composition of waste oil with high acid value is relatively complex, and the specific physical and chemical indicators of its detection are still in the stage of experimental research. It is still difficult to be accurate qualitative and quantitative.

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  • Method for detecting illegal cooking oil by using liquid chromatography-mass spectrometry (LC-MS) instrument
  • Method for detecting illegal cooking oil by using liquid chromatography-mass spectrometry (LC-MS) instrument
  • Method for detecting illegal cooking oil by using liquid chromatography-mass spectrometry (LC-MS) instrument

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Effect test

Embodiment 1

[0052] A method for detecting waste oil with LC-MS instrument, comprises the steps:

[0053] Step A, configuration of standard solution:

[0054] (1) Ammonium acetate solution: Weigh 0.3855g of ammonium acetate, add 500ml of water to dissolve, shake well, and get it;

[0055] (2) DBS standard stock solution: take about 50mg of DBS, weigh it accurately, put it in a 50ml measuring bottle, add 50% ethanol to dilute to the mark, shake well, and get it;

[0056] (3) DBS standard series solution: Take an appropriate amount of DBS standard stock solution and dilute with 50% ethanol to prepare DBS containing 4μg / ml, 10μg / ml, 20μg / ml, 50μg / ml, 100μg / ml, 500μg / ml of the standard series solution; under the chromatographic conditions constructed, accurately measure 10 μl and inject it into the LC-MS instrument, record the chromatogram, and draw the standard curve with the DBS concentration as the abscissa and the peak area as the ordinate;

[0057] Step B, sample processing:

[0058]T...

Embodiment 2

[0076] A method for detecting waste oil with LC-MS instrument, comprises the steps:

[0077] Step A, configuration of standard solution:

[0078] (1) Ammonium acetate solution: weigh 0.3g of ammonium acetate, add 500ml of water to dissolve, shake well, and get it;

[0079] (2) DBS standard stock solution: Take about 25mg of DBS, weigh it accurately, put it in a 50ml measuring bottle, add 40% ethanol to dilute to the mark, shake well, and get it;

[0080] (3) DBS standard series solution: Take an appropriate amount of DBS standard stock solution and dilute with 40% ethanol to prepare DBS containing 4μg / ml, 10μg / ml, 20μg / ml, 50μg / ml, 100μg / ml, 500μg / ml of the standard series solution; under the chromatographic conditions constructed, accurately measure 10 μl and inject it into the LC-MS instrument, record the chromatogram, and draw the standard curve with the DBS concentration as the abscissa and the peak area as the ordinate;

[0081] Step B, sample processing:

[0082] Tak...

Embodiment 3

[0100] A method for detecting waste oil with LC-MS instrument, comprises the steps:

[0101] Step A, configuration of standard solution:

[0102] (1) Ammonium acetate solution: weigh 0.4g of ammonium acetate, add 500ml of water to dissolve, shake well, and get it;

[0103] (2) DBS standard stock solution: Take about 100mg of DBS, weigh it accurately, put it in a 50ml measuring bottle, add 60% ethanol to dilute to the mark, shake well, and get it;

[0104] (3) DBS standard series solution: Take an appropriate amount of DBS standard stock solution and dilute with 60% ethanol to prepare DBS containing 4μg / ml, 10μg / ml, 20μg / ml, 50μg / ml, 100μg / ml, 500μg / ml of the standard series solution; under the chromatographic conditions constructed, accurately measure 10 μl and inject it into the LC-MS instrument, record the chromatogram, and draw the standard curve with the DBS concentration as the abscissa and the peak area as the ordinate;

[0105] Step B, sample processing:

[0106] Ta...

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Abstract

The invention relates to the technical field of illegal cooking oil detection, in particular to a method for detecting illegal cooking oil by using a liquid chromatography-mass spectrometry (LC-MS) instrument. The method comprises the following steps of: preparing a ammonium acetate solution; preparing a DBS standard stock solution; preparing DBS standard series solutions; treating a sample; constructing LC-MS chromatographic conditions; detecting the sample; judging a result and the like. The DBS is taken as a specific index for identifying the illegal cooking oil, the LC-MS instrument is used as a main detection tool, and complete detection conditions are constructed through repeated experiments; and by the method, whether the sample is illegal cooking oil can be quickly and accurately detected, the detection limit is 20mu g / ml, and defects of the current national standard are overcome.

Description

technical field [0001] The invention relates to the technical field of waste oil detection, in particular to a method for detecting waste oil with an LC-MS instrument. Background technique [0002] Waste oil is the greasy floating matter in the sewer or the leftovers and leftovers (commonly known as swill) from hotels and restaurants, which are processed and extracted by criminals through processes such as filtration, heating, precipitation, separation, decolorization, and oil washing. Oil, whose main component is still triglyceride, this kind of three-free product called waste oil is not only extremely poor in quality and extremely unhygienic, but also has many more pathogenic and carcinogenic toxic substances than real edible oil. Once the waste oil is consumed, it will destroy people's white blood cells and mucous membrane of the digestive tract, causing food poisoning and even serious consequences of cancer. Therefore, waste oil is strictly prohibited from being used in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 鲁艺王铁杰殷果关潇滢肖丽和谢普
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES