Immunohistochemical staining detection kit for colorectal cancer and application thereof

An immunohistochemistry and detection kit technology, which can be applied in measurement devices, scientific instruments, instruments, etc., and can solve the problems that the existence of tumors cannot be denied and the application scope is limited.

Inactive Publication Date: 2012-05-02
武汉康迈生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the exfoliated cells in the colorectum often have different degrees of degeneration, negative results cannot deny the existence of tumors, and the scope of clinical application of exfoliated cytology for colorectal cancer is very limited, mainly relying on histopathological examination to confirm the diagnosis

Method used

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  • Immunohistochemical staining detection kit for colorectal cancer and application thereof
  • Immunohistochemical staining detection kit for colorectal cancer and application thereof

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Experimental program
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Embodiment 1

[0044] An immunohistochemical staining kit for detecting colorectal cancer, the immunohistochemical staining kit comprising:

[0045] a) Anti-human Interleukin-17F (IL-17F) antibody derived from rabbits;

[0046] b) biotin-labeled goat anti-rabbit IgG;

[0047] c) avidin-labeled HRP;

[0048] d) normal goat serum blocking solution;

[0049] e) 3% (volume ratio) H 2 o 2 ;

[0050] f) DAB chromogenic solution;

[0051] g) hematoxylin;

[0052] b-g are products of Wuhan Boster Biological Engineering Co., Ltd.

[0053] Key in the present invention is the antibody preparation of the anti-human Interleukin-17F (IL-17F) of rabbit origin, and its method comprises the following steps:

[0054] A) Construction of IL-17F expression vector. Reagent test kit -Inducible / Secreted Kit with pCoHygro was purchased from Invitrogen. The Fc fragment tag of human immunoglobulin IgG1 was cloned into the pMT / BiP / V5-HisA expression plasmid in the kit to obtain the Drosophila expression sys...

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Abstract

The invention discloses an immunohistochemical staining detection kit for the colorectal cancer and an application thereof. The kit comprises: a, an antihuman Interleukin-17F (IL-17F) antibody from rabbit; b, biotin-labeled goat anti-rabbit IgG; c, avidin-labeled HRP; d, a normal goat serum sealant; e, 3 V% H2O2; f, a DAB coloring solution; and g, hematorylin. The detection kit which has the characteristics of high sensitivity, high specificity and high accuracy can distinguish cancerous tissues from normal tissues and also can distinguish cancerous cells from normal cells in the cancerous tissues. The IL-17F positively expresses staining in epithelial cells of normal colon tissues, but negatively expresses in the epithelial cells of the cancerous cells. The detection kit which can effectively distinguish the epithelial cells of the colorectal cancer from the normal cells provides references for the clinical diagnosis and the clinical treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to an immunohistochemical staining detection kit for colorectal cancer, and also relates to an application of the immunohistochemical staining detection kit for colorectal cancer. Background technique [0002] Colorectal cancer is one of the most common malignant tumors of the digestive tract, accounting for the third place among gastrointestinal tumors. The morbidity and mortality in our country are increasing year by year with the improvement of people's living standard and the change of diet structure. Colorectal cancer has become a disease that threatens human life and health and cannot be ignored. The onset of colorectal cancer is hidden, and the early symptoms are not obvious. Many patients are already in the advanced stage when they are diagnosed. About 25% of patients have already developed metastases at the time of initial diagnosis, and another 40%-50% of new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/531
Inventor 董晨童攒
Owner 武汉康迈生物技术有限公司
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