Method of extracting body fluid of crab

An extraction method and body fluid technology, which is applied in the field of crab body fluid extraction, can solve problems hindering research progress, and achieve the effect of simple operation, less impurities, and high protein content

Inactive Publication Date: 2012-05-09
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the study of crab body fluids, which hinders the progress of related research to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Crab body fluid extraction: Pick up the live crab with one hand, insert the needle of the syringe into the live crab with the other hand, and gently pull the core rod of the injection cylinder to make the crab body fluid flow into the injection cylinder to obtain the extracted liquid;

[0021] 2. Anticoagulant treatment: use disodium edetate as an anticoagulant, add 1 volume of 20 mmol / L disodium edetate anticoagulant solution in advance in the body fluid receiving container; After that, immediately add 1 volume of the extracted liquid obtained by extraction into the receiving container, and mix well, at this time, the concentration of disodium edetate anticoagulant in the crab body fluid is 10 mmol / L;

[0022] 3. Filtration and impurity removal treatment: Use filter paper as the filter medium to filter out the foam and impurities in the crab body fluid, and use a centrifuge to centrifuge at a speed of 4000 rpm / min for 5 minutes to settle the insoluble particles and r...

Embodiment 2

[0025] 1. Crab body fluid extraction: Pick up the live crab with one hand, insert the needle of the syringe into the live crab with the other hand, and gently pull the core rod of the injection cylinder to make the crab body fluid flow into the injection cylinder to obtain the extracted liquid;

[0026] 2. Anticoagulant treatment: use dipotassium edetate as an anticoagulant, add 1 volume of 20 mmol / L dipotassium edetate anticoagulant solution in advance in the body fluid receiving container; After that, immediately add 4 volumes of extraction liquid obtained by extraction into the receiving container, fully mix, and at this time, the concentration of dipotassium edetate anticoagulant in crab body fluid is 4 mmol / L;

[0027] 3. Filtration and impurity removal treatment: use silk cloth as the filter medium to filter out the foam and impurities in the crab body fluid, and centrifuge for 2 min at a speed of 6000 rpm / min with a centrifuge to settle the insoluble particles and remove...

Embodiment 3

[0030] 1. Crab body fluid extraction: Pick up the live crab with one hand, insert the needle of the syringe into the live crab with the other hand, and gently pull the core rod of the injection cylinder to make the crab body fluid flow into the injection cylinder to obtain the extracted liquid;

[0031] 2. Anticoagulant treatment: use disodium edetate as an anticoagulant, add 1 volume of 40 mmol / L disodium edetate anticoagulant solution in advance in the body fluid receiving container; After that, immediately add 1 volume of the extracted liquid obtained by extraction into the receiving container, and mix well, at this time, the concentration of disodium edetate anticoagulant in the crab body fluid is 20 mmol / L;

[0032] 3. Filtration and impurity removal treatment: use filter paper as the filter medium to filter out the foam and impurities in the crab body fluid, and use a centrifuge at a speed of 8000 rpm / min for 8 minutes to settle the insoluble particles and remove the sedi...

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PUM

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Abstract

The invention discloses a method of extracting body fluid of a crab, which belongs to the field of biotechnology and includes puncturing a living body of the crab with a needle of a syringe to slightly extract body fluid of the living body of the crab, immediately putting the extract into a container in which ethylenediaminetetraacetic acid anticoagulant solution or oxalate anticoagulant solution is added preliminarily, adjusting addition amount of the anticoagulant solution to control concentration of the coagulant in the extract to be within a certain range, fully mixing the anticoagulant solution with the extract prior to filtering by means of a filter medium, centrifuging the mixture at a rotation speed of 4000rpm/min-8000rpm/min to obtain clear liquor, finally adding phosphate buffer with a pH range from 5.5 to7.5 into the clear liquor to obtain the stored body fluid of the crab, and temporarily reserving the stored body fluid of the crab in a refrigerating chamber of 0 DEG C to 4 DEG C or reserving the stored body fluid of the crab for a long time at -80 DEG C to -20 DEG C. The method is simple in operation and free of expensive equipment, and the body fluid of the crab is high in protein content and has fewer impurities.

Description

technical field [0001] The invention relates to a method for extracting animal body fluid, in particular to a method for extracting crab body fluid. Background technique [0002] Shellfish paralyzing toxin (referred to as PSP toxin) is a class of derivative compounds produced by poisonous dinoflagellates or toxin-producing microorganisms and accumulated in shellfish or some fish through the food chain, with saxitoxin as the basic skeleton. Due to the wide distribution of PSP toxins and the high frequency of food poisoning caused by paralytic toxins, it has been included in the routine detection indicators for shellfish safety monitoring. [0003] Some scholars try to find antidotes through chemical drugs or immunological methods, but unfortunately, no ideal antidotes have been found so far. The research group of the inventor of the present invention has carried out anti-toxicity research on intertidal crabs such as fiery red wrinkled skin crab, blunt-toothed scull crab and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 林华娟秦小明章超桦姜沛宏
Owner GUANGDONG OCEAN UNIVERSITY
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