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Biliary excretion experimental animal model and building method

A technology of experimental animals and bile excretion, applied in the field of life sciences, to achieve the effect of simple operation process and high success rate

Inactive Publication Date: 2012-05-09
TIANJIN INSTITUTE OF PHARMA RESEARCH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant reports at home and abroad on the use of guinea pigs to establish an experimental animal model of bile duct intubation for the study of drug bile excretion

Method used

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  • Biliary excretion experimental animal model and building method
  • Biliary excretion experimental animal model and building method
  • Biliary excretion experimental animal model and building method

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Embodiment 1

[0018] The present embodiment utilizes the guinea pig bile excretion research model established by the present invention, which is suitable for preclinical pharmacokinetic research of drugs, and the specific method for establishing the model is as follows:

[0019] 1. 6 healthy guinea pigs, half male and half male, weighing 300-400g;

[0020] 2. Preparation of animal models:

[0021] (1) After the guinea pig was anesthetized (20% urethane, 1.2g / kg, i.p.), it was fixed on the fixed plate in the supine position, and the abdominal hair above and below the xiphoid process was shaved clean with a razor. After routine disinfection, use a scalpel to incise the skin longitudinally on the midline of the abdomen with the xiphoid process as the center point (the incision length is 2.5-3.0 cm). Use ophthalmic tweezers to lift the abdominal muscles, and cut the abdominal muscles with 14cm straight pointed surgical scissors. The length of the incision is about 2.5cm; Push and compress the...

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Abstract

The invention discloses a biliary excretion experimental animal model and a building method, belonging to the technical field of life science. A guinea pig is selected as an animal model; the gall bladder of the guinea pig is ligatured so that the bile directly enters into the common bile duct instead of being stored in the gall bladder after being secreted from the liver. The common bile duct is intubated so that the bile is drained to a 5ml of centrifuge tube for follow-up analysis. The model operation process is simple and quick and has high success rate; and the biliary excretion experimental animal model is an ideal experimental animal model for researching biliary excretion as far as the biotechnology medicine, such as interferon, etc and experimental animal model taking guinea pig as medical activity.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and in particular relates to a bile excretion experimental animal model and a method for establishing it. Background technique [0002] Bile excretion is one of the most important ways for most drugs and their metabolites to be excreted outside the kidney. Many important substances in the body, such as vitamins A, D, E, sex hormones, and thyroid hormones, are significantly excreted through bile. Drugs and metabolites with a molecular weight above 300 and polar groups are mainly secreted from the liver and excreted in the bile. The rate and degree of biliary excretion are affected by the physicochemical properties of the drug (molecular weight, chemical structure, polarity, etc.) and certain biological factors (animal species, age, sex, bile flow, drug biotransformation process, etc.). At the same time, due to the enterohepatic circulation of drugs in the body, the study of bile excretion ...

Claims

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Application Information

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IPC IPC(8): A61D1/00A01K67/00
Inventor 蔡永明刘昌孝张宗鹏司端运申文晋宋紫辉王海荣张骏项宗尚张春云
Owner TIANJIN INSTITUTE OF PHARMA RESEARCH
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