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Preparation method of refined poly zic and application

A technology for polymyocytes and uses, which is applied in the direction of synthetic polymer materials, active ingredients, drug combinations, anti-tumor drugs, etc.

Inactive Publication Date: 2012-06-13
TIANJIN JIMINGSHENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no relevant report on the use of polymyocytes alone as an anti-cancer adjuvant therapy. Therefore, the present invention aims at the current situation and utilizes the principle of broad-spectrum anti-virus of polymyocytes to design polymyocytes as a method that can effectively improve polymyocytes. Preparation of myocytes with antitumor effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0022] (1) Preparation of buffer:

[0023] Disodium hydrogen phosphate: 1.5g

[0024] Sodium dihydrogen phosphate: 0.2g

[0025] Water for injection 1000ml

[0026] (2) Water saturated phenol chloroform extraction:

[0027] Dissolve polyinosinic acid and polyinosinic acid with 0.5% SDS solution, prepare a 30 mg / ml solution, extract with 1 / 4 volume of water-saturated phenol and chloroform, stir at 100 r / min for 10 minutes, and then let it stand for 20 minutes minute,

[0028] Take the supernatant and add 2.5mol / L sodium acetate solution to a concentration of 0.15mol / L, mix well and then add 2 times the volume of ethanol, place at 4°C for 12 hours, centrifuge at 3000r / min for 20 minutes, and take the precipitate.

[0029] Take the precipitate and add a certain amount of PBS to dissolve it for later use.

[0030] (3) Refining of polyinosinic acid and polycytidylic acid:

[0031] The dissolved polyinosinic acid and polycytidylic acid solutions were injected into the column b...

specific Embodiment 2

[0040] (1) Preparation of buffer:

[0041] Disodium hydrogen phosphate: 1.5g

[0042] Sodium dihydrogen phosphate: 0.2g

[0043] Water for injection 1000ml

[0044] (2) Water saturated phenol chloroform extraction:

[0045] Dissolve polyinosinic acid and polyinosinic acid with 0.5% SDS solution, prepare a 30mg / ml solution, extract with 1 / 4 volume of water-saturated phenol and chloroform, stir at 100r / min for 10 minutes, and then let stand 20 minutes.

[0046] Take the supernatant and add 2.5mol / L sodium acetate solution to a concentration of 0.15mol / L, mix well and then add 2 times the volume of ethanol, place at 4°C for 12 hours, centrifuge at 3000r / min for 20 minutes, and take the precipitate.

[0047] Take the precipitate and add a certain amount of PBS to dissolve it for later use.

[0048] (3) Refining of polyinosinic acid and polycytidylic acid:

[0049] The dissolved polyinosinic acid and polycytidylic acid solutions were injected into the column bed with a syring...

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Abstract

The invention provides a preparation method of refined poly zic and application, which are characterized in that polyinosinic acid and poly zic acid are purified via a chemical method, are merged into poly zic liquor after being refined respectively, and the poly zic liquor is ultra-filtered, concentrated and added with auxiliaries, so that the refined poly zic is prepared. The refined poly zic can effectively increase an anticancer effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the preparation method and application of refined polymyocytes. Background technique [0002] Polyinosin is an interferon inducer. It produces interferon under the induction of cells in the body, which has a similar effect to interferon, so it has broad-spectrum anti-virus and immune regulation functions. Adjuvant therapy for viral infectious diseases and tumors. [0003] At present, researches on using polymyocytes as vaccine adjuvants have been carried out in China. Among them, the Chinese patent has published the patent number 93105862.7, the name is: polymyocyte complex immune adjuvant and the vaccine containing the adjuvant and the patent number is 200810011586.6, The name is: Freeze-dried vaccine and its dilution solution respectively packaged vaccine product and preparation method of the dilution solution. [0004] There are also articles titled PICA Adjuvant and PI...

Claims

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Application Information

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IPC IPC(8): A61K31/80A61P35/00
Inventor 焦月侯本晶赵钢王国新王智佳沈丽丽
Owner TIANJIN JIMINGSHENG BIOLOGICAL TECH
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