Chromatogram preparation system matched with array detector

Abstract

The invention discloses a chromatogram preparation system matched with an array detector. The chromatogram preparation system comprises the detector and a computer system connected with the detector and is characterized in that: the detector comprises a probe and an array spectrometer connected with the probe through optical fiber; and the array spectrometer receives monitored information transmitted by the probe and then transmits spectral information of the information to the computer system by adopting a full-waveband collection manner. The chromatogram preparation system matched with the array detector has the following beneficial effects that: by adopting the full-waveband collection manner, the missing of any compound arising from the wrong selection of a wavelength can be avoided; the wavelength range can be flexibly set; the absorption strengths at the same time point are automatically superposed, and weak peak forms are amplified; the data are automatically stored; the type, the purity and the concentration of biological samples can be automatically judged according to the absorption strengths under different wavelengths.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a preparative chromatographic system in chemical production. Background technique [0002] When the UV-Vis detector is used as the detector of the preparative chromatographic instrument, the preparative chromatographic software uses single wavelength or dual wavelength for monitoring. In the process of separation and purification, the compound to be separated and prepared is firstly analyzed or judged, and one or two wavelengths are selected. Based on this wavelength, it is judged whether to collect after the compound appears absorption. But if it is an unknown compound, when it is difficult to judge the absorption of this compound, the wrong wavelength is often set, and the sample does not absorb at this wavelength, which leads to the failure of the experiment; and if it is a mixed sample of multiple components, the choice of wavelength will be more difficult. Diffi...

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Problems solved by technology

But if it is an unknown compound, when it is difficult to judge the absorption of this compound, the wrong wavelength is often set, and the sample does not absorb at this wavelength, which leads to the failure of the experiment; and if it is a mixed sample of multiple components, the choice of wavelength will be more difficult. Difficult, only two wavelengths are selected, it is easy to cause some compounds to have no absorption at these two wavelengths due to too few wavelength selections, and the failure of the experiment due to the inability to present absorption peaks in the preparation collection

In the previous preparative chromatographic software, the purification method could not be better optimized due to the inability to analyze and judge the existing preparation conditions after the preparation, and the purification process was not improved much due to various restrictions in the secondary preparation.

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