Sulfamethazine enzyme-linked immunoassay kit

A technology of sulfamethazine enzyme-linked and sulfamethazine, applied in measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of cumbersome processing, lack of sensitivity and specificity of sulfamethazine, expensive instruments, etc. Achieve the effect of simple operation, overcome long operation time, and simple and easy operation

Inactive Publication Date: 2012-06-20
BIOLOGY INST OF HEBEI ACAD OF SCI
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Problems solved by technology

[0003] At present, methods for detecting SM2 include microbial methods and physical and chemical methods, such as high-pressure liquid chromatography (HPLC), gas-liquid chromatography (GLC), mass spectrometry (MS), and thin-layer chromatography (TLC), etc. The detection speed of microbial methods is relatively fast. Fast, but lacks sensitivity and specificity for sulfonamides, physical and chemical laws have the characteristics of cumbersome pretreatment, expensive instruments and professional operation, etc. It is difficult to detect a large number of samples, which is not conducive to wide-scale application

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  • Sulfamethazine enzyme-linked immunoassay kit

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Embodiment Construction

[0024] A sulfamethazine enzyme-linked immunoassay kit has a box body, and the box body includes:

[0025] (1) ELISA plate coated with sulfamethazine antigen;

[0026] (2) Sulfamethazine specific antibody;

[0027] (3) Enzyme markers;

[0028] (4) Sulfamethazine standard solution;

[0029] (5) A substrate chromogenic solution and B substrate chromogenic solution;

[0030] (6) Termination solution;

[0031] (7) concentrated washing liquid;

[0032] (8) concentrated complex solution;

[0033] (9) Concentrate the extract.

[0034] One, the preparation process of kit of the present invention is as follows:

[0035] 1. Preparation of enzyme plate coated with sulfamethazine coating agent

[0036] Dilute the sulfamethazine-coated original to 0.25 mg / ml with a carbonate buffer solution of pH 9.6, and add 100 μl / well to the wells of the microtiter plate, wherein the sulfamethazine-coated original is preferably ovalbumin as a carrier Protein; incubate at 4°C overnight or 2h at 3...

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Abstract

The invention relates to a sulfamethazine enzyme-linked immunoassay kit. A kit body comprises an elisa plate coated with sulfamethazine, a sulfamethazine specific antibody, enzyme conjugate, a sulfamethazine standard substance solution, a substrate A color-developing solution and a substrate B color-developing solution, a stop solution, concentrated washing liquid, concentrated complex liquid, and a concentrated extracting solution. The kit solves the problems of long operation time and complex pretreatment step in other kits, only 30 minutes is required by a whole operation process, and a special instrument is not required to be arranged on equipment during pretreatment; the kit can be operated easily and quickly and is suitable for field large-scale screening; and all reagents in the kit are working liquid, so the kit is easy to operate and is practicable.

Description

technical field [0001] The invention relates to a sulfamethazine ELISA detection kit. technical background [0002] Sulfamethazine (Sμlfamethazine SM2), as a kind of sulfonamide drug, has stable properties, broad antibacterial spectrum, low toxicity, easy oral absorption and low price. It is one of the most widely used and most used drugs in animal husbandry one. However, due to its long action time and metabolism time in the body, it is easy to remain in the animal body, and it may accumulate in the human body through any means. Drug accumulation concentration exceeding a certain value is harmful to human body function, and long-term accumulation will lead to the generation of sulfa drug resistance, resulting in epidemic infection of drug-resistant bacteria, and has potential carcinogenicity. Therefore, the European Union has set the maximum allowable value for sulfa drugs in milk and meat food, that is, the total amount of sulfa drugs shall not exceed 100 μg / kg, and the ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/31
Inventor 李君华吴萌李春生程华闫静辉齐颖颖
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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