Chemical emasculation seed production method based on transgenic glyphosate-resistant hybrid cotton
A glyphosate-resistant and transgenic technology, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problem of male sterility in cotton materials, save manual detasseling steps, reduce production costs, and be easy to use Effect
Inactive Publication Date: 2012-06-27
ZHEJIANG UNIV
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Problems solved by technology
During the herbicide resistance identification of transgenic herbicide-resistant germplasm, it was found that spraying herbicides at the bud stage would cause male sterility in the transgenic herbicide-resistant cotton material
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[0030] In order to verify the effect of the chemical andricide of the present invention, and the impact of the chemical andricide on the female gametes, on the basis of the previous experimental work in the laboratory, we verified the present invention again in 2009: the test was carried out at the Zhejiang University Experimental Farm, The glyphosate-resistant cotton germplasm line with the transgenic EPSPS-G6 gene cultivated in our laboratory is used as the female parent, and the chicken foot leaf (dominant trait) cotton that has been self-bred and preserved in our laboratory throughout the year is used as the male parent, which are planted adjacent to each other in Zhejiang. In the cotton experimental field of Huajiachi Campus of the University, the planting ratio of parents is 1:6. Before planting, insect-proof nets are installed in the experimental field. The isolation conditions are good and the drainage is convenient. After budding, we carried out the first chemical dest...
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The invention discloses a novel chemical emasculation seed production method based on transgenic glyphosate-resistant hybrid cotton, which is characterized in that: glyphosate-resistant gene (EPSPS-G6) transgenic glyphosate herbicide-resistant upland cotton Germplasm is adopted as a female parent, another upland cotton variety or another Germplasm is adopted as a male parent to produce the hybridized cotton combination. A seed production land which is suitable for the growth of cotton and has good isolation conditions is selected, and then the female parent and the male parent of the breeding are planted in two adjacent land blocks (planting ratio of the male and the female parents is 1:6). Glyphosate solution of 20mm is used for performing the first-time chemical emasculation on a female parent cotton plant after the female parent plant has a bud, then chemical emasculation is performed at one time every 15 days, and the chemical emasculation is performed for four times during the entire growth period. Flowers of the female parent cotton plants are sterile after 20 days of the first-time chemical emasculation, the sterility is reflected on that androecium is free from releasing powder, delays the powder release and is abnormal to release the powder, when the microspore is microscopically checked to be sterile for 100 percent; the hybrid seed production can be performed. The chemical emasculation effect is stable, the duration is long, and security and reliability can be realized.
Description
technical field [0001] The invention discloses a novel chemical maleicide seed production method based on transgenic glyphosate-resistant hybrid cotton, specifically related to hybrid cotton parents, chemical maleicide, concentration of chemical maleicide treatment, chemical maleicide time and method, Cotton chemical detasseling technology and other methods. Background technique [0002] Cotton is the most important economic crop in my country and one of the main crops that earn foreign exchange through export. With the adjustment of my country's agricultural industrial structure, the new type of agriculture with high technology, high output and high benefit is bound to replace the traditional planting industry. How to improve and further improve my country's cotton yield and quality, and increase the economic benefits of cotton planting is the most urgent task in the field of cotton science and technology. Utilization of heterosis is one of the most effective ways to incr...
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IPC IPC(8): A01H1/02
Inventor 祝水金梅磊陈进红何秋伶刘天雄叶春燕
Owner ZHEJIANG UNIV