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Preparation method of microbial organic fertilizer

A microbial organic fertilizer and mixture technology, applied in the direction of organic fertilizers, etc., can solve the problems of poor soil water and fertilizer retention capacity, threat to crop product quality, soil ecological structure damage, etc., to enhance water and fertilizer retention capacity, reduce nutrient loss, Long-lasting effect of insecticidal action

Inactive Publication Date: 2013-06-05
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, people have used a large number of chemical fertilizers and pesticides to pursue higher yields, while ignoring the protection and regulation of the soil ecological environment, resulting in the destruction of the soil ecological structure, serious land degradation, and a decline in arableability year by year. The lack or imbalance of nutrients, the reduction of beneficial microorganisms, the deterioration of soil water and fertilizer retention capacity, etc., these series of soil ecological problems pose a great threat to the improvement of crop product quality

Method used

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  • Preparation method of microbial organic fertilizer
  • Preparation method of microbial organic fertilizer

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Effect test

Embodiment 1

[0019] A preparation method of microbial organic fertilizer of the present invention, comprises the following steps:

[0020] 1. Preparation and cultivation of Phanerochaete chrysosporium bacteria liquid

[0021] (1) Strain activation: insert the parent species of Phanerochaete chrysosporium into the potato plate activation medium for activation;

[0022] (2) Dissolve the Phanerochaete chrysosporium spores after activation in sterile water to make a bacterial suspension for subsequent use, and the number of spores contained in it is 10 4 a / mL~10 6 a / mL;

[0023] (3) Inoculate the prepared Phanerochaete chrysosporium suspension into Kirk inorganic culture medium, inoculate 3mL of Phanerochaete chrysosporium suspension per 100mL culture medium, and then inoculate the mixture at 39°C, 120r / min Under shaking culture for 72h;

[0024] 2, take by weighing decomposed rapeseed cake fertilizer (the mass ratio of nitrogen, phosphorus, potassium is 3.5: 1: 1) 20Kg, add the Phanerocha...

Embodiment 2

[0029] A preparation method of microbial organic fertilizer of the present invention, comprises the following steps:

[0030] 1. Preparation and cultivation of Phanerochaete chrysosporium bacteria liquid

[0031] (1) Strain activation: insert the parent species of Phanerochaete chrysosporium into the potato plate activation medium for activation;

[0032] (2) Dissolve the Phanerochaete chrysosporium spores after activation in sterile water to make a bacterial suspension for subsequent use, and the number of spores contained in it is 10 4 a / mL~10 6 a / mL;

[0033] (3) Inoculate the prepared Phanerochaete chrysosporium suspension into Kirk inorganic culture medium, inoculate 4mL of Phanerochaete chrysosporium suspension per 100mL culture medium, and then inoculate the mixed solution at 36°C, 150r / min condition Under shaking culture for 70h;

[0034] 2, take by weighing decomposed rapeseed cake fertilizer (the mass ratio of nitrogen, phosphorus, potassium is 3.5: 1: 1) 20Kg, a...

Embodiment 3

[0040] The microbial organic fertilizer (T 1 ), the microbial organic fertilizer (T 2 ) is used as basal fertilizer or hole fertilizer, applied to tobacco field production, covered immediately after being applied, the dosage per mu is 20Kg, microbial organic fertilizer does not contact chemical fertilizer, does not mix with chemical fertilizer; Under the condition that keeps other conditions all identical, A comparative experiment was carried out in the treated tobacco field with only the application of decomposed rapeseed cake fertilizer as the control sample (CK). Soil samples were collected at different growth stages of tobacco, including cluster stage (30d after transplanting), blooming stage (50d), topping stage (57d), lower leaf mature stage (69d), middle leaf mature stage (93d), upper leaf mature stage Leaf maturation stage (110d), measure soil enzyme activity (collect the fresh soil of each growth stage of tobacco, utilize 3,5-dinitrosalicylic acid colorimetric method...

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Abstract

The invention discloses a preparation method of microbial organic fertilizer, which comprises the following steps: inoculating Phanerochaete chrysosporium solution in rotten rapeseed cake fertilizer, and carrying out solid culture; mixing bentonite, Bacillus thuringlensis wettable powder and the obtained rapeseed cake fertilizer growing Phanerochaete chrysosporium, and air-drying to obtain the microbial organic fertilizer. The preparation method of the invention is simple and convenient, the prepared fertilizer has a soil improvement effect, and can regulate soil nutrient supply through regulating soil microenvironment, and improve fertilizer utilization rate and prolong fertilizer effect.

Description

technical field [0001] The invention relates to the field of fertilizers, in particular to a method for preparing microbial organic fertilizers. Background technique [0002] Soil is a vital part of the human food chain and the basis of agricultural production systems. It is not only an important medium for crop growth but also maintains crop productivity, affecting the quality of the environment and the health of plants and even humans. However, for a long time, people have used a large number of chemical fertilizers and pesticides to pursue higher yields, while ignoring the protection and regulation of the soil ecological environment, resulting in the destruction of the soil ecological structure, serious land degradation, and a decline in arable capacity year by year. The lack or imbalance of nutrients, the reduction of beneficial microorganisms, the deterioration of soil water and fertilizer retention capacity, etc., these series of soil ecological problems have posed a g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08
Inventor 尚翠陈桂秋曾光明胡日生曾中楚轶欧易百科李欢可官嵩贺建敏陈安伟周颖邹正军
Owner HUNAN UNIV
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