Method for enhancing production intensity of producing itaconic acid by fermentation method

A technology for producing strength and itaconic acid, applied in the field of fermentation engineering, can solve problems such as lack of a good solution, and achieve the effects of shortening the fermentation cycle, significant effect, and improving production strength

Inactive Publication Date: 2012-06-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to shorten the fermentation cycle and increase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: The fermentation conditions are the same as those of the control example, and the initial pH, carbon source, nitrogen source, and inorganic salt of the seed medium are optimized by single factor and orthogonal experiment methods.

[0021] It was found that the age of the inoculated bacteria remained unchanged before and after optimization, but the dry weight of the bacteria increased from the initial 2.3g / L to 7.4g / L, an increase of 3.2 times.

Embodiment 2

[0022] Example 2: The fermentation conditions are the same as those of the control example, and the initial pH, carbon source, nitrogen source, and inorganic salts of the fermentation medium are optimized by single factor and orthogonal experiment methods.

[0023] It was found that: ①After optimization, the fermentation period was shortened from 120h to 48h, shortened to 40% of the original; ②The dry weight of bacteria in the fermentation broth increased from 3.9g / L to 9.8g / L, which was 2.5 times the original; ③The fermentation broth The content of itaconic acid increased to 65g / L, and the production intensity was 1.35g / (L·h).

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PUM

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Abstract

The invention discloses a method for enhancing the production intensity of producing an itaconic acid by a fermentation method, which belongs to the technical field of fermentation engineering. According to the method for enhancing the production intensity of producing the itaconic acid by the fermentation method, fermentation optimization is performed by taking Aspergillus terreus FMME 033 as a production strain for producing the itaconic acid by utilizing a single-factor experiment method and an orthogonal experiment method so as to determine the inoculation cell age to be 36h and the fermentation period to be 48h, and determine the best compositions of a seed culture medium and a fermentation culture medium. According to the method for enhancing the production intensity of producing the itaconic acid by the fermentation method, the production intensity of the itaconic acid is effectively enhanced to 1.35g/(L h), and a new reference is provided for producing the itaconic acid by the fermentation method.

Description

technical field [0001] The invention relates to a method for increasing the production intensity of itaconic acid produced by fermentation, which belongs to the field of fermentation engineering. Background technique [0002] Itaconic acid, also known as methylene succinic acid, is an unsaturated dibasic organic acid with a wide range of uses. It can be seen from the structural formula that there is an unsaturated double bond and two active carboxyl groups in the molecule. , and the carbon-carbon double bond is in a conjugated relationship with a carbonyl group, so it has a strong reactivity. Using its two carboxyl groups, it can be used as a monomer of unsaturated polyester; using its C-C double bond, it can be copolymerized with a variety of substituted olefins to form a polymer with hydroxyl functions, making it suitable for use in chemical fibers and synthetic resins. , plastics, rubber, pharmaceuticals, coatings, surfactants, water treatment, lubricating oil additives ...

Claims

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Application Information

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IPC IPC(8): C12P7/44C12R1/66
Inventor 刘立明高倩刘杰陈坚
Owner JIANGNAN UNIV
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