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Human miR-328 antisense nucleic acid and application thereof

An antisense, nucleotide technology, applied in the field of biomedical materials, can solve the problems of poor curative effect, poor curative effect, and insignificant improvement of tumor patient survival rate in patients with distant metastasis, and achieve the ability to inhibit growth and malignant proliferation. Effect

Inactive Publication Date: 2012-07-04
SUZHOU GENEPHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the past 30 years, although the comprehensive treatment of tumors has been very common clinically, the comprehensive treatment based on surgery and supplemented by radiotherapy and chemotherapy has not significantly improved the survival rate of cancer patients, and the 5-year overall survival rate is still low, hovering At about 30% to 55%, there is no significant improvement, and the 5-year survival rate of middle and advanced patients is even lower, about 20%.
Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Human miR-328 antisense nucleic acid and application thereof
  • Human miR-328 antisense nucleic acid and application thereof
  • Human miR-328 antisense nucleic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Detection of inhibitory activity of miR-328 antisense oligonucleotides on human glioma cell line U87 / MG

[0033] First, miR-328 antisense oligonucleotide was synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd., the sequence is: 5'-ACGGAAGGGCAGAGAGGGCCAG-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0034] Cell culture:

[0035] U87 / MG cells (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences), cultured in 10% FBS-DMEM medium (FBS was purchased from Gibco, DMEM was purchased from Hyclone), 37 ° C, 5% CO 2 to cultivate. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0036] Transfection:

[0037] 1) One day before transfection, inoculate cultured cells in a 96-well plate with an appropriate amount of culture medi...

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Abstract

The invention discloses antisense oligonucleotide for suppressing expression of human microRNA-328, and application of the antisense oligonucleotide. The antisense oligonucleotide is specifically combined to the human miR-328, comprises sequences complementary with at least 13 continuous nucleotides in a 5'-CUGGCCCUCUCUGCCCUUCCGU-3' nucleotide sequence, particularly a 5'-ACGGAAGGGCAGAGAGGGCCAG-3' sequence. The antisense oligonucleotide can be ribonucleotide, deoxyribonucleotide or chimera of ribonucleotide and deoxyribonucleotide, can modify any nucleotide in a chain. The miR-328 antisense oligonucleotide can effectively suppress the expression of miR-328 in human glioma cells, and suppress the cells from growing and proliferating to effectively treat human glioma and other tumors with high miR-328 expression.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to a use of microRNAs (miRNA), in particular to human microRNA-328 (human miR-328) antisense nucleic acid and its application. The antisense nucleic acid can be complementary to human miR-328, thereby inhibiting the expression of human miR-328 to play an anti-tumor effect. The invention also relates to a pharmaceutical composition containing the miRNA antisense nucleic acid. Background technique [0002] miRNAs are small non-coding RNAs, 20-25bp in length, usually transcribed by RNA polymerase II (Pol II), and generally the initial product is a large one with a cap structure (7MGpppG) and a polyA tail (AAAAA) The pri-miRNA. These pri-miRNAs are processed into pre-miRNA precursor products consisting of 70 nucleotides under the action of RNase III Drosha and its cofactor Pasha. RAN-GTP and exportin 5 transport th...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P25/00
Inventor 丁侃张佩琢段春晓李捷沈孝坤
Owner SUZHOU GENEPHARMA
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