Single nucleotide polymorphism sequence of dairy cattle FGF2 (Fibroblast Growth Factor 2) gene and detection method of single nucleotide polymorphism sequence

A technology of single nucleotide polymorphism and detection method, applied in the field of single nucleotide polymorphism sequence of dairy cow FGF2 gene and its detection field, can solve the problem of few researches on the correlation between reproductive traits and milk production traits, etc. The effect of improving the survival rate

Inactive Publication Date: 2012-07-04
NORTHWEST A & F UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few studies on the correlation between FGF2 gene and reproductive traits and milk production traits of dairy cows at home and abroad

Method used

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  • Single nucleotide polymorphism sequence of dairy cattle FGF2 (Fibroblast Growth Factor 2) gene and detection method of single nucleotide polymorphism sequence
  • Single nucleotide polymorphism sequence of dairy cattle FGF2 (Fibroblast Growth Factor 2) gene and detection method of single nucleotide polymorphism sequence
  • Single nucleotide polymorphism sequence of dairy cattle FGF2 (Fibroblast Growth Factor 2) gene and detection method of single nucleotide polymorphism sequence

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Effect test

Embodiment 1

[0025] Example 1: Detection method and identification of SNP site of FGF2 gene

[0026] (1) Determination steps of the SNP site (i.e. the A / G mutation of the first intron 11646 site) of the FGF2 gene of the present invention:

[0027] The method for determining the SNP site in the first intron of the FGF2 gene of the present invention comprises the steps of:

[0028] (1) Collect the blood, milk and semen of 2773 dairy cows, extract the genomic DNA of the cows from the blood, milk and semen respectively, and use the GFX Genomic DNA Purification Kit to extract (the operation steps are carried out according to the operating instructions of the kit), and the individual cows Sample collection and sources are shown in Table 1;

[0029] Table 1 Sample collection and source

[0030]

[0031] (2) Use Nanodrop to measure the concentration of genomic DNA, extract the genomic DNA (100ng / μL) of 50 individuals with the same concentration, take 4 μL for each individual, the total volu...

Embodiment 2

[0051] Embodiment 2: Association analysis between the single nucleotide polymorphism sequence genotype of FGF2 gene and character

[0052] The A / G SNP site of the first intron 11646 site of the FGF2 gene of the present invention, after identifying a total of 2773 individual genotypes from 3 different Holstein cow populations, utilizes the following model to carry out the relationship between genotypes and traits Correlation Analysis:

[0053] the y ijkl =μ+s i +mgs j +d ijk τ+f l +ε ijkl

[0054] In the formula, y ijkl is the milk protein yield, milk protein rate, milk fat content and milk fat rate, and production and service life of the k-th daughter of the i-th son of j male animal; mgs j is the random effect of j sire; τ represents the infection effect of Mycobacterium paratuberculosis; d ijk is an index of variation that is represented by values ​​0 or 1 for hypothetically infected and non-infected cattle, respectively; f l Indicates the effect of the three gen...

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Abstract

The invention relates to a single nucleotide polymorphism sequence of a dairy cattle FGF2 (Fibroblast Growth Factor 2) gene and a detection method of the single nucleotide polymorphism sequence, belonging to the technical field of genetic engineering. The single nucleotide polymorphism sequence of the dairy cattle FGF2 gene comprises a single nucleotide polymorphism sequence of which the 11646th site of a first intron of the dairy cattle FGF2 gene is A or G. The detection method comprises the following steps of: with dairy cattle genome DNA as a template and a primer pair P as a primer, carrying out PCR (Polymerase Chain Reaction) amplification on the dairy cattle FGF2 gene; and sequencing a purified PCR product to determine that the single nucleotide polymorphism 11646th site of the dairy cattle FGF2 is A or G. According to the single nucleotide polymorphism sequence, the SNP (Single nucleotide Polymorphism) site of the dairy cattle FGF2 gene is related to butterfat production, butterfat percentage, somatic cell count and reproductive capacity; and found through genotyping an externally-fertilized embryo, the SNP site is related to the embryo survival rate; and through association analysis study on FGF2 genetic polymorphism and character, the SNP site can be used for dairy cattle molecular mark assisted breeding and increase of the externally-fertilized embryo survival rate.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a single nucleotide polymorphism sequence of a dairy cow FGF2 gene and a detection method thereof. Background technique [0002] FGF2 is a member of the fibroblast growth factor family and regulates the growth and development of the body through the FGF receptor (FGFR) on the cell surface. Signal transduction induced by FGF2 is necessary for normal cell growth and differentiation, and participates in physiological processes such as angiogenesis, embryonic development, and bone formation. In addition, studies have shown that FGF2 can also induce ES cells to differentiate into the nervous system in some cases. The fibroblast growth factor (FGF) family regulates mammary gland development in bovines at different physiological stages, and FGF2 is expressed in the endometrium of bovine estrus and early pregnancy, which can increase the mRNA and protein of troph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/68C12N15/11
Inventor 王昕张智英张婷婷李托赵志东徐华荣
Owner NORTHWEST A & F UNIV
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