Predominant bacteria and their spectrum in the microbial flora of Pu-erh tea

Abstract

The invention relates to a molecular identification method for a predominant flora structure in the pile-fermentation process of Pu'er tea. The molecular identification method comprises the following steps of: 1) taking Pu'er tea subjected to different pile-turning frequencies at different positions in the pile-fermentation process as samples to be detected; 2) extracting total DNA from the samples to be detected, and purifying; 3) performing polymerase chain reaction (PCR) amplification by taking the total DNA of the samples to be detected as a template under the guide of special primers, and recovering and purifying target fragments; 4) performing denatured gradient gel electrophoresis (DGGE) on the purified target fragments, and dyeing to obtain a DGGE fingerprint; 5) recovering predominant bands from the gel, performing PCR amplification by taking the predominant bands as templates under the guide of an upstream primer 338F and a downstream primer R518, and recovering, purifying and sequencing the target fragments; and 6) comparing sequencing results in the step 5) with a sequence of a standard flora to determine the predominant florae in the pile-fermentation process of the Pu'er tea.

Description

technical field [0001] The invention relates to an identification method for identifying the dominant bacterial flora structure in the Pu'er tea stack fermentation process, special primers and DGGE fingerprint spectrum. Background technique [0002] Pu-erh cooked tea is a post-fermentation made from the sun-dried green tea of ​​Yunnan's unique big-leaf tea [Camellia sinensis (Linn) var. tea. Microbes play an important role in the process of fermenting Pu-erh tea. Without microorganisms, the quality and flavor of Pu-erh tea cannot be guaranteed. This invention studies the dominant microbial flora in Pu-erh tea fermented by stacking and An identification method is provided. If these dominant flora are used in the stacking fermentation production process of Pu'er tea, it will be beneficial to the production of Pu'er tea and improve the quality. [0003] At present, the research on microorganisms in fermented Pu-erh tea mainly uses traditional methods such as separation, purif...

AI Technical Summary

Problems solved by technology

[0006] However, the chemical and physical properties of Pu-erh tea fermented in piles and urban sewage plant sludge and farmland soil are highly heterogeneous, and the number and population distribution of microorganisms are also significantly different. has a difference

At present, there are no relevant reports on the microbial community composition of compost-fermented Pu-erh tea using PCR-DGGE technology at home and abroad

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