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Fluorescence spectrum correction method and fluorescence spectrum measuring device

A technology of fluorescence spectrum and calibration method, which is applied in the field of fluorescence spectrum separation to achieve high accuracy and eliminate overlap.

Active Publication Date: 2016-04-06
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Fluorescence spectrum correction method and fluorescence spectrum measuring device
  • Fluorescence spectrum correction method and fluorescence spectrum measuring device
  • Fluorescence spectrum correction method and fluorescence spectrum measuring device

Examples

Experimental program
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Effect test

no. 1 approach

[0034] Example of Fluorescence Spectral Correction Method Not Using Single Stained Samples

no. 2 approach

[0036] Example of Fluorescence Spectrum Measuring Device Not Using Single-stained Sample

[0037] 1. First Embodiment

[0038] Correction method

[0039]First, a fluorescence spectrum correction method (hereinafter, simply referred to as a correction method) according to the first embodiment of the present invention will be described. In the correction method of this embodiment, when the fluorescence spectra acquired from microparticles labeled with a plurality of fluorescent dyes are separated for each dye, the previously measured fluorescence spectra are used as reference spectra.

[0040] Here, "microparticle" broadly includes biomimetic microparticles such as cells, microorganisms, and liposomes, or synthetic particles such as latex particles, gel particles, and industrial particles. Bionic particles include: chromosomes that constitute various cells, liposomes, mitochondria, organelles (cell organs), and the like. Cells include: plant cells, animal cells, blood cells, ...

Embodiment

[0064] Hereinafter, advantages of the present invention will be described in detail with reference to embodiments of the present invention. In this example, the measurement date, the potential of the detector, the type of coupled antibody, and the type of microparticles were varied, the fluorescence spectra were compared, and the difference was investigated.

[0065] In this example, Immuno-TROL (manufactured by Beckman Coulter Co., Ltd.) or Multi-Check (manufactured by Becton Dickinson Co., Ltd.), which are commercially available as precise regulatory cells, were used as samples. They are positive processing controls for flow cytometry (whole blood control check target object) and represent scattered light, distribution of cell populations, fluorescence intensity, and antigen density since the positive rate and absolute number of specific surface antigens are Calibrated in single cells. A commercially available product (manufactured by Beckman Coulter Co., Ltd. or Becton Dic...

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Abstract

The invention discloses a fluorescence spectrum correction method and a fluorescence spectrum measurement device. A fluorescence spectrum correction method comprising: comparing a fluorescence spectrum acquired from a microparticle labeled with a plurality of fluorescent dyes with a reference spectrum, thereby separating the fluorescence spectrum into a fluorescence spectrum for each dye, and converting the previously measured spectrum The data are used as reference spectra.

Description

technical field [0001] The invention relates to a fluorescence spectrum correction method and a fluorescence spectrum measurement device. Specifically, the present invention relates to a technique for separating, for each pigment (pigment), fluorescence spectra obtained from microparticles labeled with a plurality of fluorescent dyes. Background technique [0002] In general, when analyzing microparticles such as cells, microorganisms, and liposomes, flow cytometry (flow cytometry) (for example, see the second edition published by Shujunsha Co., Ltd. on August 31, 2006, edited by Hiromitsu Nakauchi) is used. "CellEngineeringAdditionalVolumeExperimentProtocolSeriesFlowCytometryFreely"). Flow cytometry is a method of analyzing a plurality of particles one by one by irradiating particles flowing in a row in a flow path with laser light (excitation light) of a specific wavelength and detecting fluorescence or scattered light emitted by the particles. In flow cytometry, light d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N15/1429G01N21/6428G01N21/274G01N15/01
Inventor 角田正也二村孝治酒井启嗣加藤泰信
Owner SONY CORP