Salt-tolerant gene CcSOS1 and application thereof

A salt-tolerant gene and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem that it is difficult to obtain excellent salt-tolerant varieties

Inactive Publication Date: 2012-07-11
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the complexity of plant salt-tolerant traits, it is difficult to obtain excellent salt-tolerant varieties by traditional breeding methods

Method used

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  • Salt-tolerant gene CcSOS1 and application thereof
  • Salt-tolerant gene CcSOS1 and application thereof
  • Salt-tolerant gene CcSOS1 and application thereof

Examples

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Effect test

Embodiment 1

[0058] 1. Construction of plant expression vector pCAMBIA 1301-CcSOS1

[0059] The total RNA was extracted from the halophytic plant Chrysanthemum daisylum, and 1 μg of total RNA was reverse-transcribed into cDNA according to the M-MLV reverse transcription kit (TaKaRa), the cDNA product was digested with RNase, and primers were designed using the cDNA as a template A PCR reaction was carried out, and SalI and KpnI restriction sites were respectively introduced into the upstream and downstream of the CcSOS1 gene. The upstream primer was: CcSOS1-Sal-F: GGGTCGAC ATG GGA TCG GTG GCA AAC AAC GT (SEQ ID NO.2), and the downstream primer was CcSOS1-Kpn-R: GGGGTACC TTA GGG AGC TCG GGG GA (SEQ ID NO. 3). 50 μL reaction system: 5.0 μL 10×PCR Buffer, 1.0 μL (20 μmol L-1) each of CcSOS1-Sal-F and CcSOS1-Kpn-R primers, 4.0 μL (2.5 mmol L-1) of dNTP mix, PrimeSTARTM HS DNA Polymerase 0.2 μL, cDNA template 1 μL, ddH 2 O 37.8 μL; reaction program: pre-denaturation at 95°C for 4 min, then me...

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Abstract

The invention belongs to the field of plant gene engineering and transgenosis breeding, and discloses a salt-tolerant gene CcSOS1 and an application thereof. The nucleotide sequence of the salt-tolerant gene CcSOS1 is SEQ ID No.1. The salt-tolerant gene CcSOS1 can be used for improving the salt resistance of chrysanthemum grandiflorum. A method for improving the salt resistance of the chrysanthemum grandiflorum comprises the following steps of: (1) building a plant expression vector of the CcSOS1 gene; and (2) transplanting the CcSOS1 gene-containing plant expression vector into chrysanthemum morifolium by virtue of sonication-assisted agrobacterium-mediated transformation, and cultivating to firstly obtain a resistant plant. According to the invention, the new salt-tolerant gene CcSOS1 is firstly cloned from halophyte dendranthema crassum, and a high salt-tolerant transgenosis chrysanthemum grandiflorum strain is obtained by means of high salt experimental analysis through integrating the gene into chrysanthemum grandiflorum genome. According to the method, the salt resistance of the chrysanthemum grandiflorum can be observably improved.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering and transgenic breeding, and relates to a salt-tolerant gene CcSOS1 and its application. Background technique [0002] Chrysanthemum (Chrysanthemum grandiflorum), a perennial herb of the Compositae family, among which cut chrysanthemum is one of the four major cut flowers in the world. Chrysanthemum is native to my country, because nature endows it with characteristics such as rich color, changeable flower pattern and easy cultivation, making it It is deeply loved by the people. In recent years, cut chrysanthemum has occupied a large proportion in cut flower consumption, and its cultivated area has ranked first in my country's cut flower production. As the global soil salinization continues to increase, the production of chrysanthemum is facing serious challenges. Traditional physical and chemical methods to modify soil not only cost a lot of money, but also aggravate the secondary saliniz...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N15/84A01H5/00
Inventor 陈素梅安娟陈发棣蒋甲福宋爱萍卢军刚管志勇刘兆磊房伟民
Owner NANJING AGRICULTURAL UNIVERSITY
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