Molecular biological method for distinguishing striped rice borer from chilotraea auricilia dudgeon

Abstract

The invention relates to a molecular biological method for distinguishing striped rice borer from chilotraea auricilia dudgeon and belongs to the technical field of biology. The method comprises the following steps of: taking a genome DNA (deoxyribonucleic acid) as a template, carrying out PCR (polymerase chain reaction) amplification by use of specific primers, namely F: 5'-GGGCAAGAAAGTAGACGCCTGGTT-3'and R: 5'-AAAACCCAAAGAGGCGACACGGTA-3', and carrying out enzyme digestion on an amplified product by use of restriction enzyme MspA1 I, wherein the detected sample is explained to be the striped rice borer if only one segment with the length of 400bp is obtained; and the detected sample is explained to be the chilotraea auricilia dudgeon if two segments with the lengths of 247bp and 153bp areobtained. The method is high in stability, short in period, and high in sensitivity, and is specially used for distinguishing the striped rice borer from the chilotraea auricilia dudgeon with high sensitivity and quick detection.

Description

1. Technical field [0001] The invention relates to a molecular biological distinction method for rice pests Chilo suppressalis walker and Taiwan rice borer (Chilo auricilia Dudgeo), belonging to the field of biotechnology. 2. Background technology [0002] Chilo borer and Taiwan rice borer are frequent pests on rice. Because they are both boring pests, they are difficult to control and often cause large-scale production reduction of rice. [0003] The damage methods of the rice stem borer and the Taiwan rice borer are similar. The larvae first damage the leaf sheath, and then invade the rice stem through the borehole of the leaf sheath. The damage to rice is also very similar, with symptoms such as withered sheath, dead heart, and dry booting. According to the damage in the paddy field, we cannot accurately determine whether the damage is caused by the rice borer or the Taiwan rice borer. The larvae of Chilo suppressalis and Taiwan rice borer are usually 6th instar. The bod...

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Problems solved by technology

[0002] Chilo borer and Taiwan rice borer are common pests on rice. Since they are both boring pests, they are difficult to control and often cause large-scale production reduction of rice.

Furthermore, when the stem borer or Taiwan rice borer is infected by microorganisms, the insect body completely loses its original morphological characteristics, making it impossible to determine what kind of borer it is

[0004] At present, the identification of Chilo suppressalis and Taiwan rice borer is usually distinguished by morphological characteristics, which must ensure that the key morphological characteristics of the sample to be tested are intact, but as mentioned above, when the morphological characteristics are lost, it cannot be accurately identified. Distinguish between Chilo suppressalis and Taiwan rice borer, which greatly limits research in related fields of Chilo suppressalis and Taiwan rice borer

For example, if you want to study the microbial infection status of Chilo borer or Taiwan rice borer in a certain area, but because you can't accurately distinguish what kind of borer is the sample infested by microorganisms, so the research in this field is greatly limited

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