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Blood coagulation factor and fibrinolysis measuring method

A blood coagulation factor and measurement method technology, which is applied in the field of determination of blood coagulation factors and D-dimer, can solve problems such as inability to measure other items, and achieve the effects of ensuring consistency, saving consumable costs, and easy operation

Active Publication Date: 2013-08-14
BEIJING PRECIL INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent only measures plasma viscosity and cannot measure other items

Method used

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  • Blood coagulation factor and fibrinolysis measuring method
  • Blood coagulation factor and fibrinolysis measuring method
  • Blood coagulation factor and fibrinolysis measuring method

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Embodiment 1

[0029] The assay method of blood coagulation factor of the present invention and D-dimer uses a semi-automatic blood coagulation analyzer to test, and assay step is:

[0030] A. Collect the patient's blood sample, perform anticoagulant treatment on the blood sample, generate anticoagulant whole blood, and centrifuge the anticoagulant whole blood at a speed of 3000 rpm, and keep it for 15-30 minutes (the preferred time is 20 minutes) , or the centrifugal force is 1000g, keep it for 15-30 minutes (the preferred time is 20 minutes), separate the plasma, and put it into the plasma storage cup for later use;

[0031] Collect the patient's blood sample (according to the detection standard dosage, usually 5ml), anticoagulate the blood sample to generate anticoagulant whole blood, and centrifuge the anticoagulant whole blood; Coagulant 3.8% (concentration) sodium citrate for anticoagulation, the ratio of anticoagulant to blood is 1:9; inject anticoagulant whole blood into the sample t...

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Abstract

The invention relates to a blood coagulation factor and fibrinolysis measuring method, which is used for testing by using a semi-automatic blood coagulation analyzer. The blood coagulation factor and fibrinolysis measuring method comprises the following testing steps of: collecting a patient blood sample and separating blood plasma out; injecting the blood plasma into a testing cup by using a sample feeding gun; putting the testing cup in a blood coagulation factor measuring device, adding a reagent, starting a timing button of the blood coagulation factor testing device, and testing the blood plasma in the testing cup by the blood coagulation factor testing device according to a double magnetic circuit magnetic bead method; and injecting the blood plasma into a fibrinolysis testing cup by the sample feeding gun, putting the fibrinolysis testing cup in a fibrinolysis testing device, adding a reagent, starting a timing button of the fibrinolysis testing device, testing the blood plasmain the fibrinolysis testing cup by the fibrinolysis testing device according to a photoelectric turbidimetry, and printing and outputting fibrinolysis data after the testing by the fibrinolysis testing device is ended. The blood coagulation factor and fibrinolysis measuring method disclosed by the invention is simple and convenient and greatly reduced in cost.

Description

technical field [0001] The invention relates to a method for measuring blood coagulation factors and D-dimer. A semi-automatic blood coagulation analyzer is used to measure blood coagulation factors according to a double magnetic circuit magnetic bead method, and D-dimer is measured according to a photoelectric turbidimetric method. Background technique [0002] Chinese patent 200520103130.4 discloses a method for testing blood plasma using a fully automatic hemorheometer. The first scheme: ① close the valve 17, ② start the work of the suction pump 19, suck the quantitative test sample, ③ open the valve 17, and let the test sample Free flow, ④ detect the time of the test sample flowing from the sensor 17 to the sensor 18, ⑤ compare the time used with the time of pure water flowing out, which is the plasma viscosity. The second solution: ①Use the sensor to detect the liquid level of the test plasma, and then let the sample needle penetrate into the liquid surface for a certai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49G01N21/27G01N21/17
Inventor 陈生肖继昌邵卫峰
Owner BEIJING PRECIL INSTR CO LTD
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