Method for obtaining dendrobium aphyllum seeds to germinate effective symbiotic fungi

Abstract

The invention discloses a method for obtaining dendrobium aphyllum seeds to germinate effective symbiotic fungi. The method comprises the following steps of: subjecting parent dendrobium aphyllum plants to artificial cross-pollination to obtain mature seeds, putting the seeds into a special seed bag, placing the seed bag at an origin place where dendrobium aphyllum plants grow, and inducing the seeds under natural conditions to germinate and produce protocorms; cultivating the obtained protocorms in an artificial culture medium under sterile conditions after surfaces of the protocorms are sterilized, inducing the growth of endophytic fungi in the protocorms, and purifying the endophytic fungi to obtain pure bacterial colonies after hyphae grow out; and cultivating the obtained fungi and the dendrobium aphyllum seeds for symbiotic germination, setting a control experiment so as to test effectiveness of the obtained fungi to the germination of the dendrobium aphyllum seeds, screening the symbiotic fungi which are effective to germinate the dendrobium aphyllum seeds, subjecting the fungi to molecular identification, and storing the fungi. According to the method disclosed by the invention, a new way is found for the cultivation of seedlings through the symbiotic germination of the dendrobium aphyllum seeds and the fungi.

Description

technical field [0001] The invention relates to a method for obtaining effective symbiotic fungi for seed germination of Orchidaceae plants, in particular to a method for inducing seed germination in the original place of Dendrobium aphyllum to obtain protocorms, which are then separated, cultivated and screened out from the protocorms. Effective symbiotic fungal approach to seed germination. Background technique [0002] The seeds of orchids are very small, with only immature embryos. Under natural conditions, seed germination requires specific symbiotic fungi to obtain nutrients. At present, the germination of Orchidaceae seeds mostly uses artificial medium for non-symbiotic germination under sterile conditions. Although the germination rate is high, when the endangered species are returned to the wild, the survival rate of the seedlings transplanted to the natural environment is low, and due to The inability to establish a symbiotic relationship with fungi in nature resu...

AI Technical Summary

Problems solved by technology

However, due to the existence of a large number of endophytic fungi with unknown functions in the roots of orchids, the screening and isolation of effective fungi for seed germination become complicated and cumbersome.

At the same time, it is still uncertain whether the fungi in the roots of different orchids are the same as the effective symbiotic fungi in the seed germination stage.