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Method for preparing fine dioscin and application of fine dioscin

A slender diosgenin and the technology of the technical scheme are applied in the field of medicine and can solve the problems of complex extraction and separation methods, small preparation amount and low pertinence.

Active Publication Date: 2012-08-01
YUNNAN INST OF MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The reported extraction and separation methods for fine diosgenin are complex, the preparation volume is small, and the pertinence is not high.

Method used

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  • Method for preparing fine dioscin and application of fine dioscin
  • Method for preparing fine dioscin and application of fine dioscin
  • Method for preparing fine dioscin and application of fine dioscin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Weigh 50 kg of Nanzhonglou medicinal materials and put them into the extraction tank with interlayer; measure 180 liters of 80% ethanol and add them to the extraction tank, soak the medicinal materials for 50 minutes, then pass steam into the interlayer to heat up to 80 ° C, keep warm Reflux extraction for 2 hours, release the extract; add 150 liters of 80% ethanol to the medicinal dregs, reflux extraction for 1 hour, release the extract; combine the two extracts, concentrate and recover the ethanol, release the residual liquid into a stainless steel barrel, and there will be precipitation Precipitate. After standing for 6 hours, filter under reduced pressure to obtain a filter cake, dry and pulverize the filter cake to obtain a fine diosgenin crude product. Dissolve the crude product in chloroform / methanol, filter, concentrate the filtrate until turbidity appears, stop the concentration, let it crystallize, filter under reduced pressure after 8 hours, dry and pulverize...

Embodiment 2

[0073] Weigh 50 kg of Nanzhonglou medicinal materials and put them into the extraction tank with interlayer; measure 180 liters of 80% methanol and add them to the extraction tank, soak the medicinal materials for 50 minutes, then pass steam into the interlayer to heat up to 70 ° C, keep warm Reflux extraction for 2 hours, release the extract; add 150 liters of 80% methanol to the medicinal dregs, reflux extraction for 1 hour, release the extract; combine the two extracts, concentrate and recover methanol, release the residual liquid into a stainless steel barrel, and there will be precipitation Precipitate. After standing for 6 hours, filter under reduced pressure to obtain a filter cake, dry and pulverize the filter cake to obtain a fine diosgenin crude product. Dissolve the crude product in methanol / water, filter, concentrate the filtrate until turbidity appears, stop concentrating, leave to crystallize, filter under reduced pressure after 8 hours, dry and pulverize the fil...

Embodiment 3

[0076] Weigh 50 kg of Nanzhonglou medicinal materials and put them into the extraction tank with interlayer; measure 180 liters of 80% ethanol and add them to the extraction tank, soak the medicinal materials for 50 minutes, then pass steam into the interlayer to heat up to 80 ° C, keep warm Reflux extraction for 2 hours, release the extract; add 150 liters of 80% methanol to the medicinal dregs, reflux extraction for 1 hour, release the extract; combine the two extracts, concentrate and recover methanol, release the residual liquid into a stainless steel barrel, and there will be precipitation Precipitate. After standing for 6 hours, filter under reduced pressure to obtain a filter cake, dry and pulverize the filter cake to obtain a fine diosgenin crude product. Dissolve the crude product in methanol / water, filter, concentrate the filtrate until turbidity appears, stop concentrating, leave to crystallize, filter under reduced pressure after 8 hours, dry and pulverize the filt...

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Abstract

The invention relates to a method for preparing fine dioscin and an application of the fine dioscin to antitumor aspect. The preparation method comprises the following steps of: weighing medicinal materials, putting into an extracting tank, soaking the medicinal materials with a water-containing organic solvent of which the amount is 3-12 times (v / w) that of the medicinal materials for 30-100 minutes, heating to 40-100 DEG C, refluxing or extracting with other auxiliary methods for 1-8 hours, draining an extracting solution, and extracting for 2-5 times; concentrating and recovering the solvent, draining a residual liquid, standing, precipitating a precipitate out, filtering, and drying and smashing a filter cake to obtain a crude product; and repeatedly crystalizing the crude product with the solvent to obtain a pure product of which the purity is over 95 percent. As proved by results of in-vitro and in-vivo antitumor tests on the fine dioscin, the fine dioscin prepared with the method has an antitumor effect, and particularly has better effects on the aspect of inhibition of human intestinal cancers, human kidney cancers, human prostatic cancers and human mammary cancer tumor cells. The fine dioscin prepared with the method can be prepared into antitumor tablets, capsules, injections, oral liquids and the like.

Description

technical field [0001] The invention relates to a method for preparing fine diosgenin and the application of its preparation in antitumor, belonging to the field of medicine. Background technique [0002] Dioscorea saponin is a steroidal saponin natural compound, which was first extracted and isolated from Dioscorea gracillima by Japanese scholar Tsukamoto Takeo in 1954. Afterwards, scholars continued to isolate it from plants of Dioscoreaceae, Dioscorea, Liliaceae Pachyphylla, Liliaceae Astragalus, Liliaceae Polygonatum and other genera. And its physiological activity has been studied in many aspects, and it is found that the compound has certain physiological activities in anti-tumor, antibacterial, parasite killing, hemolysis, blood lipid lowering, anti-osteoporosis, and anti-mutation. The existing method for preparing fine diosgenin is basically a laboratory process: as reported by Tsukamoto Takeo et al. in Yakugaku Zasshi, 1954, 74: 1127, the fine dioscin is obtained f...

Claims

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Application Information

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IPC IPC(8): C07J71/00A61K31/7048A61P35/00
Inventor 梅双喜苏梅何雷吴德松崔涛朱兆云王京昆孙敏
Owner YUNNAN INST OF MATERIA MEDICA
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