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Method for inducing retinal stem cells to differentiate into photosensory cells

A stem cell differentiation and photoreceptor cell technology, applied in the field of inducing retinal stem cells to differentiate into photoreceptor cells, can solve the problems of mixed mature cell types, unfavorable clinical research and clinical application of RSCs induced differentiation into photoreceptor cells, and achieve simple operation and high differentiation efficiency Effect

Inactive Publication Date: 2013-12-25
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

At present, extensive research work has been carried out on the mechanism of retinal stem cells (RSCs) directional differentiation into photoreceptor cells at home and abroad, and many substances and related signaling pathways that promote the differentiation of RSCs into photoreceptor cells have been described, such as gene silencing and transduction. Infection, etc., but always face such a problem: under the condition of promoting differentiation, the differentiation of RSCs into photoreceptor cells increases, but due to the introduction of viral genes and the implementation of genetic manipulation, there are problems in the safety of in vivo research and the introduction of RSCs to other types of cells However, RSCs isolated from retinas that have differentiated mature cells (late embryonic, adult retina, etc.) will also be mixed with other mature cell types, which will not be conducive to in vivo experiments in which RSCs are induced to differentiate into photoreceptor cells , clinical research and clinical application

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  • Method for inducing retinal stem cells to differentiate into photosensory cells
  • Method for inducing retinal stem cells to differentiate into photosensory cells

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Embodiment Construction

[0017] The present invention will be described in detail below in conjunction with the embodiments and drawings, which are only for illustration and not limiting the scope of application of the present invention. The present invention is not limited to the following embodiments or examples, and any modifications and variations that do not violate the spirit of the present invention shall be included within the scope of the present invention.

[0018] The method for inducing retinal stem cells to differentiate into photoreceptor cells in this embodiment is to induce retinal stem cells to differentiate into photoreceptor cells by inhibiting the IGFIRα receptor of retinal stem cells.

[0019] In this embodiment, the retinal stem cells are optic cup retinal stem cells, and of course they can also be retinal stem cells from adult retina, late embryonic retina, etc., all of which can achieve the purpose of the invention, but the effect of using optic cup retinal stem cells is better....

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Abstract

The invention discloses a method for inducing retinal stem cells to differentiate into photosensory cells, which includes inhibiting IGF IR alpha (insulin-like growth factor I receptor alpha) of a retinal stem cell so as to lead the retinal stem cell to differentiate into photosensory cells. Compared with the prior art, the method introduces no virus gene and needs no genetic manipulation, cross contamination of retinal mature cells is avoided, and the method is high in differentiation efficiency and simple to operate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for inducing retinal stem cells to differentiate into photoreceptor cells. Background technique [0002] Retinitis pigmentosa (RP) is one of the most common causes of hereditary blindness. Since the cause of persistent vision loss in RP patients is the degeneration and apoptosis of photoreceptor cells, which cannot regenerate, transplantation of healthy retinal tissue or photoreceptor cells has become the most promising therapeutic strategy. At present, extensive research work has been carried out on the mechanism of retinal stem cells (RSCs) directional differentiation into photoreceptor cells at home and abroad, and many substances and related signaling pathways that promote the differentiation of RSCs into photoreceptor cells have been described, such as gene silencing and transduction. Infection, etc., but always face such a problem: under the condition of promoting dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071
Inventor 黄小勇谭小玲阴正勤
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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