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Application of Y-STR (Y chromosome-short tandem repeat)

A Y-STR, individual technology, applied in the biological field, can solve the problems of difficult genetic relationship and low mutation rate of Y-STR, and achieve the effect of improving the resolution

Inactive Publication Date: 2012-08-01
GENESKY BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem now is that there are relatively few commonly used STR sites. The commonly used ones are DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS425, DYS426, DYS434, DYS435, DYS436, DYS437, DYS439, DYS, and these The general mutation rate of Y-STR is low, and it is difficult to deeply and comprehensively reflect the genetic relationship between individuals in the population. Screen and obtain a large number of Y-STRs with high mutation rate for human origin, evolution, ethnic groups, and individuals. The study of genetic relationships is very important

Method used

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  • Application of Y-STR (Y chromosome-short tandem repeat)
  • Application of Y-STR (Y chromosome-short tandem repeat)
  • Application of Y-STR (Y chromosome-short tandem repeat)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 screened out 36 new Y-STR sites

[0030] 1. Prediction of Y-STR

[0031] (1) Checked and sorted out STR information in biological public databases, and obtained 201 STR information reported by NCBI (as of 2011-11);

[0032] (2) Bioinformatics predicts STR, and obtains 128 Y-STR sites with repeat units repeated more than 10 times;

[0033] (3) Compare the number of repetitions of these STR sites in the three genomes (refercese, HuRef, Celera) in the NCBI database, and obtain information on STR sites with large differences in the number of repetitions;

[0034] (4) Combined with the literature, biological databases and bioinformatics predictions, according to the sequence characteristics of the actual Y-STRs, the reported sites with DYS numbers were removed, and 103 Y-STRs predicted by bioinformatics were screened out, named as They are respectively named S1, S2...S128, S128 according to the size of their positions on the Y chromosome. The 36 new Y-STR loci s...

Embodiment 2

[0059] Example 2 Clarify the genetic relationship by detecting 36 Y-STRs

[0060] Thirty-nine male samples were collected from different regions, some of whom were close relations such as father and son, uncle and nephew. By detecting the 36 Y-STRs screened out in Example 1 above, their genetic relationship was clarified.

[0061] The results of the data show that through these 36 newly discovered Y-STR loci, the relative relationship between father and son or uncle and nephew in these 39 male samples from different regions is clearly clarified (see Table 2 on the next page). The common Y-STR loci -STR is difficult to distinguish between uncles and nephews or individuals who are relatively close relatives. In this aspect, the detection accuracy of the 36 Y-STR sites newly discovered by the present invention is several orders of magnitude higher than that of the commonly used Y-STR sites.

[0062] Table 2 Y-STR genetic polymorphism data analysis of 39 male samples

[0063] N...

Embodiment 3

[0065] Example 3 Individual identification by detecting 36 Y-STRs

[0066] Collect random 150 male samples (the samples come from the male samples of random physical examination in Shanghai Ren'ai Hospital), and use the 36 Y-STR new sites of the present invention to detect whether it can achieve the purpose of distinguishing each person.

[0067] The experimental results show that in 150 male samples, 150 haplotypes were found (see the test data of 150 random samples in Table 3 below for detailed haplotype information), which can 100% distinguish the collected random samples, further proving that The 36 Y-STRs newly discovered by the present invention have a very high degree of discrimination.

[0068] Table 3 Test data of 150 random samples

[0069]

[0070]

[0071] Note: In Table 3, because the samples come from random physical examination samples, the sample numbers follow the hospital numbers.

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Abstract

The invention discloses application of Y-STR in individual identification. The Y-STR is selected from any one of or a combination of over any two of DYG1-DYG36. The invention also discloses a detection kit and a detection method used for individual identification. The invention applies 36 new Y-STR loci with high mutation rates effectively in individual identification, and the resolution and accuracy are substantially raised.

Description

technical field [0001] The invention belongs to the field of biotechnology and is applied to biological science research and clinical molecular diagnosis, in particular to the application of a Y-STR (Y chromosome short tandem repeat sequence). Background technique [0002] The human genome includes the nuclear genome consisting of 22 pairs of autosomes and 1 pair of sex chromosomes and the cytoplasmic genome composed of mitochondrial DNA. The vast majority of human genetic information is inherited from both parents, and each generation will undergo gene recombination, but there are two segments of DNA in the genome that are exceptions, representing the mitochondrial DNA inherited from the mother and the Y chromosome representing the inheritance from the father. Inherited from only one parent, recombination rarely occurs. In this way, the genetic mutations in different periods are faithfully recorded, and it is a very good historical recorder. This is an important tool for ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 姜正文彭冬铂李才华
Owner GENESKY BIOTECH INC
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