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Kit for immunohistochemical staining detection of colorectal cancer and application

An immunohistochemistry and detection kit technology, applied in the biological field, can solve the problems of limited application scope and cannot deny the existence of tumors, and achieve the effect of reducing immune rejection.

Inactive Publication Date: 2012-08-01
武汉康迈生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the exfoliated cells in the colorectum often have different degrees of degeneration, negative results cannot deny the existence of tumors, and the scope of clinical application of exfoliated cytology for colorectal cancer is very limited, mainly relying on histopathological examination to confirm the diagnosis

Method used

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  • Kit for immunohistochemical staining detection of colorectal cancer and application
  • Kit for immunohistochemical staining detection of colorectal cancer and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] An immunohistochemical staining kit for colorectal cancer, comprising:

[0048] a) mouse-derived anti-human MUC1 monoclonal antibody;

[0049] b) biotin-labeled goat anti-mouse IgG;

[0050] c) avidin-labeled HRP;

[0051] d) normal goat serum blocking solution;

[0052] e) 3% (volume ratio) H 2 o 2 ;

[0053] f) DAB chromogenic solution;

[0054] g) hematoxylin;

[0055] b-g are products of Wuhan Boster Biological Engineering Co., Ltd.

[0056] Antibody preparation of mouse-derived anti-human MUC1, the steps are:

[0057] A) Mice were immunized with antigenic peptides of rat anti-CD19 (eBioscience) and human MUC1 (Applied Biosystems, Foster City, CA), and the mice were immunized every other week for a total of 3 times, and the passively immunized mice were collected Serum, the anti-MUC1 response titer was determined by ELISA method.

[0058] B) Take spleen cells from mice that have received passive immunization and produced high-titer anti-MUC1 responses, fus...

Embodiment 2

[0061] A kit for immunohistochemical staining detection of colorectal cancer, the kit comprising:

[0062] a) mouse-derived anti-human MUC1 monoclonal antibody;

[0063] b) biotin-labeled goat anti-mouse IgG;

[0064] c) avidin-labeled HRP;

[0065] d) normal goat serum blocking solution;

[0066] e) 3% (volume ratio) of H 2 o 2 ;

[0067] f) DAB chromogenic solution;

[0068] g) hematoxylin;

[0069] The detection steps are:

[0070] 1. The tissue was embedded in paraffin and sectioned. After the paraffin section was placed in a 60°C oven for 1 hour, it was dewaxed to water, and washed three times with PBS (pH7.4), 5 minutes each time.

[0071] 2. Antigen microwave repair: 10mM pH 6.0±0.1 sodium citrate buffer solution is poured into the microwave repair box, put the slice into the repair solution, and heat in a Haier brand ordinary microwave oven to ensure that the repair temperature is 95-98 ° C, and avoid boiling as much as possible , can take the repair method of...

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Abstract

The invention discloses a kit for immunohistochemical staining detection of colorectal cancer and application. The kit comprises (1) mouse-derived anti-human MUC1 (Mucin 1) monoclonal antibody, (b) biotin-labeled goat anti mouse IgG (Immunoglobulin G); (c) avidin-labeled HRP (Horseradish Peroxidase); (d) normal goat serum confining liquid; (e) 3vol% H2O2; (f) DAB (Diaminobenzidine) development liquid; and (g) brazilein. The detection has the characteristics of high sensitivity, high specificity and high accuracy, not only can distinguish canceration tissue and normal tissue, but also can distinguish canceration cells and normal cells in the canceration tissue. MUC1 is in positive expression staining in the canceration cell epithelia, and is negatively expressed in the epithelia of the normal cola tissue, so that the canceration epithelia and normal cells of the cola and recta can be effectively distinguished, and reference is provided for clinical diagnosis and treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to an immunohistochemical staining detection kit for colorectal cancer, and also relates to an application of the immunohistochemical staining detection kit for colorectal cancer. Background technique [0002] Colorectal cancer is one of the most common malignant tumors of the digestive tract, accounting for the third place among gastrointestinal tumors. The morbidity and mortality in our country are increasing year by year with the improvement of people's living standard and the change of diet structure. Colorectal cancer has become a disease that threatens human life and health and cannot be ignored. The onset of colorectal cancer is hidden, and the early symptoms are not obvious. Many patients are already in the advanced stage when they are diagnosed. About 25% of patients have already developed metastases at the time of initial diagnosis, and another 40%-50% of new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577C07K16/18
Inventor 严俊
Owner 武汉康迈生物技术有限公司