Tissue culture propagation method for Polygala fallax Hemsl

Abstract

The invention relates to the technical field of plant tissue culture and provides a tissue culture propagation method for Polygala fallax Hemsl. By utilizing the method, a large amount of tissue culture seedlings of Polygala fallax Hemsl can be obtained in a short term, which effectively solves the problems of low yield, difficulty in harvest and severe resource shortage caused by limited wild resources and the like of the Polygala fallax Hemsl, and lays a foundation for scale production of high-quality nursery seedling of the Polygala fallax Hemsl and resource cultivation and utilization. The invention mainly comprises the following steps of: (1) collection and sterilization of an explant; (2) induction of adventitious bud; (3) enrichment culture of adventitious bud; (4) rooting culture of adventitious bud, and (5) hardening-seedling and transplantation.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and propagation of the lotus chrysanthemum. Background technique [0002] Polygala fallax Hemsl. is a plant of the genus Polygala in the family Polygalaceae. In places such as Hunan, Fujian, Guangxi, Jiangxi, Guangdong and Yunnan, it grows in sparse forests on hillsides or in jungles in valleys. Chrysanthemum chrysanthemum is a precious Chinese herbal medicine, and its whole herb is used for medicine; it has the effects of tonifying, strengthening, dispelling dampness, and dispelling blood stasis. It has anti-inflammatory and anti-lipid effects, and it has the effects of enhancing immunity and hypoxia resistance in mice. [0003] The medicinal value of chrysanthemum chrysanthemum has long been recognized by the people. In addition, in recent years, its medicinal value has been paid more and more attention, and the market demand is large...

AI Technical Summary

Problems solved by technology

[0005] Therefore, in view of the above-mentioned problems, the present invention provides a method for tissue culture and propagation of chrysanthemum chrysalis, which can obtain a large amount of chrysanthemum tissue culture seedlings in a short period of time, effectively solving the problem of low output of chrysanthemum chrysalis and harvesting. Harvesting difficulties, limited wild resources lead to severe shortage of resources, etc., and at the same time lay the foundation for the large-scale production of high-quality seedlings and even the cultivation and utilization of resources