Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device
A honeycomb and honeybee technology, applied in the field of ecotoxicity testing, can solve the problems of vulnerability to health of test personnel, increase of the cost of anesthesia test, and slow down of the test process, so as to speed up the implementation progress, improve the operability, and reduce the cost. Effect
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Embodiment 1
[0027] Environmental conditions for bee anesthesia: indoor temperature: 25°C; 2 Pressure: 0.1Mpa, a total of 117 bees to be anesthetized. continuous CO 2 After 5 min, stop feeding CO 2 , the recovery rate of anesthetized bees was 3.41% after 5 minutes; the recovery rate was 71.8% after 8 minutes; the recovery rate was 100% after 10 minutes.
Embodiment 2
[0029] Adopt the environmental condition of honeybee anesthesia in example 1 and the CO of feeding 2 Under stress conditions, a total of 115 bees were to be anesthetized. Pass CO 2 After 10 min, stop feeding CO 2, the recovery rate of anesthetized bees was 0% after 5 minutes of recovery; 1.74% after 10 minutes; 49.6% after 15 minutes; 98.3% after 20 minutes, and 100% after 25 minutes.
Embodiment 3
[0031] Adopt the environmental condition of honeybee anesthesia in example 1 and the CO of feeding 2 Under stress conditions, a total of 128 bees were to be anesthetized. Pass CO 2 After 15 min, stop feeding CO 2 , the recovery rate of anesthetized bees was 0% after 5 minutes; 0.86% after 10 minutes; 23.5% after 15 minutes; 86.4% after 20 minutes, and 99.6% after 25 minutes.
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