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Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device

A honeycomb and honeybee technology, applied in the field of ecotoxicity testing, can solve the problems of the health of the test personnel, the increase of the cost of anesthesia test, and the slow down of the test process, so as to speed up the implementation progress, improve the operability, and reduce the cost. Effect

Inactive Publication Date: 2014-06-04
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method of utilizing the high toxicity of materials, high environmental hazards, time-consuming and labor-consuming, increased anesthesia test cost, easy slowdown of the test process, and vulnerable health of test personnel in the current honeybee anesthesia method. Method for honeybee colony anesthesia with honeybee spleen anesthesia device

Method used

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  • Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Environmental conditions for bee anesthesia: indoor temperature: 25°C; 2 Pressure: 0.1Mpa, a total of 117 bees to be anesthetized. continuous CO 2 After 5 min, stop feeding CO 2 , the recovery rate of anesthetized bees was 3.41% after 5 minutes; the recovery rate was 71.8% after 8 minutes; the recovery rate was 100% after 10 minutes.

Embodiment 2

[0029] Adopt the environmental condition of honeybee anesthesia in example 1 and the CO of feeding 2 Under stress conditions, a total of 115 bees were to be anesthetized. Pass CO 2 After 10 min, stop feeding CO 2, the recovery rate of anesthetized bees was 0% after 5 minutes of recovery; 1.74% after 10 minutes; 49.6% after 15 minutes; 98.3% after 20 minutes, and 100% after 25 minutes.

Embodiment 3

[0031] Adopt the environmental condition of honeybee anesthesia in example 1 and the CO of feeding 2 Under stress conditions, a total of 128 bees were to be anesthetized. Pass CO 2 After 15 min, stop feeding CO 2 , the recovery rate of anesthetized bees was 0% after 5 minutes; 0.86% after 10 minutes; 23.5% after 15 minutes; 86.4% after 20 minutes, and 99.6% after 25 minutes.

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Abstract

The invention discloses a method for carrying out bee colony anaesthesia by utilizing a bee honeycomb anaesthesia device. The method comprises the following steps of: taking a honeycomb from a bee box, and placing the honeycomb in a honeycomb groove so as to place in a box body; introducing carbon dioxide in the box body through a guide tube, exhausting air in the box body, and moving a collecting tank and the honeycomb groove into the box body completely, wherein the honeycomb groove is subjected to local anaesthesia through carbon dioxide introduced in the box body; and stopping introducing the carbon dioxide after bees on the honeycomb are in full anaesthesia, shivering the bees to the collecting tank, and taking out anesthetic bees so as to be used for a bee biologic test. The method provided by the invention overcomes the defects that the number of the anesthetic living bodies is small and the pollution is easy to generate in the conventional method; the method has the characteristics that anaesthesia on bees can be carried out safely in batches, the anaesthesia quantity is large, the processing cost is low, the operability is strong and the like; and the method is suitable for a zoology poisonousness testing test of a great deal of bees, and is a preparing method of a zoology poisonousness testing test bee living body which is high efficiency, safe and economical.

Description

technical field [0001] The invention belongs to the field of ecotoxicity testing, and in particular relates to an improved honeybee honeycomb anesthesia test device and a honeybee honeycomb colony anesthesia method using the device. Background technique [0002] In the process of observing and studying the active insects of bee species, it is often necessary to perform close-range operations on them. Anesthesia and other pre-treatments on bees will greatly facilitate the development of close-range operations in the later stage. Currently available anesthesia methods There are mainly ether anesthesia and carbon dioxide anesthesia. The use of ether anesthesia to anesthetize active insects such as bees requires the operator to have good operating experience and strictly control the amount of ether used, and ether is easy to cause certain damage to the tested insects, which will affect the accuracy of later test results. Excessive use of ether will also pollute the indoor envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 卜元卿焦少俊沈飞单正军陈源周军英韩志华程燕
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA