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Antisense oligodeoxyncleotide of human miR-1913 and application thereof

An oligonucleotide and nucleotide technology, applied in the field of biomedical materials, can solve the problems of insignificant improvement in the survival rate of tumor patients, poor curative effect in patients with distant metastasis, and low survival rate in patients with advanced disease. Good inhibitory effect, anti-malignant proliferation ability, long half-life effect

Active Publication Date: 2012-08-22
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0008] In the past 30 years, although the comprehensive treatment of tumors has been very common clinically, the comprehensive treatment based on surgery and supplemented by radiotherapy and chemotherapy has not significantly improved the survival rate of cancer patients, and the 5-year overall survival rate is still low, hovering At about 30% to 55%, there is no significant improvement, and the 5-year survival rate of middle and advanced patients is even lower, about 20%.
Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Antisense oligodeoxyncleotide of human miR-1913 and application thereof
  • Antisense oligodeoxyncleotide of human miR-1913 and application thereof
  • Antisense oligodeoxyncleotide of human miR-1913 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] Example: Detection of antisense oligonucleotides of human miR-1913 on human glioma cell line U87 / MG inhibitory activity

[0031] First, the miR-1913 antisense oligonucleotide was synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd., the sequence is: 5'-UGGCAGCAGCGGAGGGGGCAGA-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0032] Cell culture:

[0033] U87 / MG cells (human brain astroglioblastoma, purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences) were purchased from Gibco in 10% FBS-DMEM medium (FBS (fetal bovine serum), DMEM (a A regular medium) purchased from Hyclone), at 37 ° C, 5% CO 2 cultivated under conditions. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0034] Transfection:

[0035] 1) One day before transfectio...

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Abstract

The invention discloses an antisense oligodeoxyncleotide for inhibiting the expression of human microRNA-1913 and the application thereof. The antisense oligodeoxyncleotide is specifically bound to human miR-1913, and contains 13-22 continuous nucleotide complemented sequences among the following nucleotide sequence: 5'-UCUGCCCCCUCCGCUGCUGCCA-3', particularly the sequence: 5'-UGGCAGCAGCGGAGGGGGCAGA-3'. The antisense oligodeoxyncleotide of the invention may be a ribotide, a deoxyribotide, or a chimera of a ribotide and a deoxyribotide, and can modify any nucleotide in a chain. The antisense oligodeoxyncleotide of miR-1913 of the invention can effectively inhibit miR-1913 expression in human glioma cell, and can inhibit the growth and propagation of the human glioma cell, thereby effectively treating glioma and other tumours of miR-1913 high expression.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to a microRNAs (miRNA) antisense oligonucleotide, in particular to human microRNA-1913 (human miR-1913) antisense oligonucleotide and applications thereof. The antisense oligonucleotide can be complementary to human miR-1913, thereby inhibiting the expression of human miR-1913 to play an anti-tumor effect. The present invention also relates to a pharmaceutical composition comprising the miRNA antisense oligonucleotide. Background technique [0002] miRNAs (also known as microRNA or microRNA) are small non-coding RNAs with a length of 20-25bp, usually transcribed by RNA polymerase II (Pol II), and generally the initial product is a large cap structure (7MGpppG) and pri-miRNA (primary miRNA, primary miRNA) of polyadenylic acid tail (AAAAA). These pri-miRNAs are processed into precursor miRNAs (precursormiRNA, pre-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P25/00
Inventor 丁侃张佩琢李捷沈孝坤
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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