Antisense oligonucleotide for human miR-431 and application thereof

A technology of oligonucleotides and ribonucleotides, applied in microRNAs antisense oligonucleotides, human microRNA-431 antisense oligonucleotides and its application fields, can solve the problem of patients with distant metastasis Poor curative effect, low survival rate of middle and advanced patients, no significant improvement, etc., to achieve the effect of inhibiting malignant proliferation, long half-life, and small side effects

Active Publication Date: 2015-06-17
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the past 30 years, although the comprehensive treatment of tumors has been very common clinically, the comprehensive treatment based on surgery and supplemented by radiotherapy and chemotherapy has not significantly improved the survival rate of cancer patients, and the 5-year overall survival rate is still low, hovering At about 30% to 55%, there is no significant improvement, and the 5-year survival rate of middle and advanced patients is even lower, about 20%.
Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Antisense oligonucleotide for human miR-431 and application thereof
  • Antisense oligonucleotide for human miR-431 and application thereof
  • Antisense oligonucleotide for human miR-431 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Example: Detection of antisense oligonucleotides of human miR-431 on human glioma cell line U87 / MG inhibitory activity

[0030] First, miR-431 antisense oligonucleotide was synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd., the sequence is: 5'-UGCAUGACGGCCUGCAAGACA-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0031] Cell culture:

[0032] U87 / MG cells (human brain astroglioblastoma, purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences), in 10% FBS-DMEM medium (FBS (fetal bovine serum) were purchased from Gibco, DMEM (a A regular medium) purchased from Hyclone), at 37 ° C, 5% CO 2 nourish. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0033] Transfection:

[0034] 1) One day before transfection, inoculate cultured cell...

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Abstract

The invention discloses an antisense oligonucleotide for inhibiting the expression of human micro RNA-431, and the application of the antisense oligonucleotide. The antisense oligonucleotide is combined with the human miR-431 in a way of specific binding, and comprises 13-21 continuous nucleotide complementary sequences in the following nucleotide sequence of 5'-UGUCUUGCAGGCCGUCAUGCA-3', and especially comprises the sequence of 5'-UGCAUGACGGCCUGCAAGACA-3'. The antisense oligonucleotide can be ribonucleotide, deoxyribonucleotide or chimera between the ribonucleotide and the deoxyribonucleotide, and can be used for modifying any nucleotide in the chain. The antisense oligonucleotide can effectively inhibit the expression, growth and multiplication of miR-431 in human brain glioma cells, thus effectively treating brain glioma and other tumours with high miR-431 expression.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to a microRNAs (miRNA) antisense oligonucleotide, in particular to human microRNA-431 (human miR-431) antisense oligonucleotide and applications thereof. The antisense oligonucleotide can be complementary to human miR-431, thereby inhibiting the expression of human miR-431 to play an anti-tumor effect. The present invention also relates to a pharmaceutical composition comprising the miRNA antisense oligonucleotide. Background technique [0002] miRNAs (also known as microRNA or microRNA) are small non-coding RNAs with a length of 20-25bp, usually transcribed by RNA polymerase II (Pol II), and generally the initial product is a large cap structure (7MGpppG) and pri-miRNA (primary miRNA, primary miRNA) of polyadenylic acid tail (AAAAA). These pri-miRNAs are processed into precursor miRNAs (precursormiRNA, pre-miRN...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P25/00
Inventor 丁侃张佩琢李捷沈孝坤
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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