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High-density fermentation medium for recombinant chicken interferon alpha and preparation method thereof

A technology for high-density fermentation and chicken interferon, which is applied in the field of high-density fermentation medium for recombinant chicken interferon alpha and its preparation, can solve problems such as the inhibitory effect of recombinant bacteria, achieve high-density growth, reduce production costs, and prepare simple method effect

Active Publication Date: 2016-01-27
天津市圣世莱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the high-density fermentation media for the production of recombinant chicken interferon-alpha contain glucose as the carbon source, and acetic acid is easily produced in the early stage of culture, resulting in the inhibitory effect on the growth and expression of recombinant bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A high-density fermentation medium for recombinant chicken interferon α, mainly composed of glucose, peptone, yeast powder, KH 2 PO 4 、K 2 HPO 4 、Na 2 HPO 4 12H 2 O, (NH 4 ) 2 SO 4 , NH 4 Cl, MnSO 4 ·5H 2 O, CoCl 2 ·6H 2 O, Na 2 MoO 4 2H 2 O, ZnCl 2 、CuSO 4 ·5H 2 O, H 3 BO 4 , FeSO 4 ·7H 2 O, CaCl 2 2H 2 O, MgSO 4 ·7H 2 O, defoamer and H 2 O composition, including glucose 8g, peptone 5g, yeast powder 5g, KH 2 PO 4 3g,K 2 HPO 4 2g, Na 2 HPO 4 12H 2 O3g, (NH 4 ) 2 SO 4 0.8g, NH 4 Cl0.2g, MnSO 4 ·5H 2 O0.005g, CoCl 2 ·6H 2 O0.01g, Na 2 MoO 4 2H 2 O0.003g, ZnCl 2 0.005, CuSO 4 ·5H 2 O0.005g, H 3 BO 4 0.004g, FeSO 4 ·7H 2 O0.01g, CaCl 2 2H 2 O0.01, MgSO 4 ·7H 2 O0.6g, defoamer 0.2g, H 2 O1L.

[0024] The above-mentioned preparation method for the high-density fermentation medium of recombinant chicken interferon α, the specific preparation steps are as follows:

[0025] (1) Accurately weigh the components of the speci...

Embodiment 2

[0030] A high-density fermentation medium for recombinant chicken interferon α, mainly composed of glucose, peptone, yeast powder, KH 2 PO 4 、K 2 HPO 4 、Na 2 HPO 4 12H 2 O, (NH 4 ) 2 SO 4 , NH 4 Cl, MnSO 4 ·5H 2 O, CoCl 2 ·6H 2 O, Na 2 MoO 4 2H 2 O, ZnCl 2 、CuSO 4 ·5H 2 O, H 3 BO 4 , FeSO 4 ·7H 2 O, CaCl 2 2H 2 O, MgSO 4 ·7H 2 O, defoamer and H 2 O composition, including glucose 10g, peptone 6g, yeast powder 6g, KH 2 PO 4 2g,K 2 HPO 4 1g, Na 2 HPO 4 12H 2 O2g, (NH 4 ) 2 SO 4 0.6g, NH 4 Cl0.1g, MnSO 4 ·5H 2 O0.001g, CoCl 2 ·6H 2 O0.004g, Na 2 MoO 4 2H 2 O0.001g, ZnCl 2 0.001, CuSO 4 ·5H 2 O0.001g, H 3 BO 4 0.002g, FeSO 4 ·7H 2 O0.01g, CaCl 2 2H 2 O0.01, MgSO 4 ·7H 2 O0.4g, defoamer 0.1g, H 2 O1L.

[0031] The above-mentioned preparation method for the high-density fermentation medium of recombinant chicken interferon α, the specific preparation steps are as follows:

[0032] (1) Accurately weigh the components of the spe...

Embodiment 3

[0037] A high-density fermentation medium for recombinant chicken interferon α, mainly composed of glucose, peptone, yeast powder, KH 2 PO 4 、K 2 HPO 4 、Na 2 HPO 4 12H 2 O, (NH 4 ) 2 SO 4 , NH 4 Cl, MnSO 4 ·5H 2 O, CoCl 2 ·6H 2 O, Na 2 MoO 4 2H 2 O, ZnCl 2 、CuSO 4 ·5H 2 O, H 3 BO 4 , FeSO 4 ·7H 2 O, CaCl 2 2H 2 O, MgSO 4 ·7H 2 O, defoamer and H 2 O composition, including glucose 10g, peptone 5g, yeast powder 5g, KH 2 PO 4 3g,K2 HPO 4 3g, Na 2 HPO 4 12H 2 O4g, (NH 4 ) 2 SO 4 0.6g, NH 4 Cl0.1g, MnSO 4 ·5H 2 O0.006g, CoCl 2 ·6H 2 O0.01g, Na 2 MoO 4 2H 2 O0.001g, ZnCl 2 0.001g, CuSO 4 ·5H 2 O0.007g, H 3 BO 4 0.006g, FeSO 4 ·7H 2 O0.01g, CaCl 2 2H 2 O0.02g, MgSO 4 ·7H 2 O0.6g, defoamer 0.1g, H 2 O1L.

[0038] The above-mentioned preparation method for the high-density fermentation medium of recombinant chicken interferon α, the specific preparation steps are as follows:

[0039] (1) Accurately weigh the components of the spec...

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Abstract

The invention provides a high-density fermentation culture medium for recombination chicken interferon alpha and a preparation method thereof. The high-density fermentation culture medium for recombination chicken interferon alpha is mainly composed of glucose, peptone, yeast powder, KH2PO4, K2HPO4, Na2HPO4-12H2O, (NH4)2SO4, NH4Cl, MnSO4-5H2O, CoCl2-6H2O, Na2MoO4-2H2O, ZnCl2, CuSO4-5H2O, H3BO4, FeSO4-7H2O, CaCl2-2H2O, MgSO4-7H2O, defoamer and H2O. The components are dissolved in distilled water, the pH value is adjusted by ammonia water, and finally the culture medium is prepared in a fermentation tank in sterilization mode. The fermentation culture medium reduces the inhibitory effect on thallus growing and protein expression in the fermentation process in the prior art, achieves high-density growing of recombination escherichia coli and improves yield of the recombination chicken interferon alpha.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a high-density fermentation medium for recombinant chicken interferon alpha and a preparation method thereof. Background technique [0002] Interferon is a kind of glycoprotein with antiviral activity on the same kind of cells. It achieves preventive and therapeutic effects by inhibiting the replication of viral DNA and RNA. It is an important component for vertebrates to resist virus, bacteria, parasite infection and tumor invasion . At the same time, interferon also has various biological functions such as nerve, immunity, and endocrine regulation. Since lsaacs and Lindenmann discovered interferon in 1957, interferon has shown strong antiviral and immunomodulatory activities and broad application prospects. Therefore, the study of interferon has attracted more and more attention from people. Compared with traditional pet health treatment drugs, interferon has shown gre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C12R1/19
Inventor 高应瑞
Owner 天津市圣世莱科技有限公司
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