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Method for simultaneously preparing indocalamus leaf volatile oil, total flavone and total polysaccharide

A technology of volatile oil and total flavonoids is applied in the field of biological active ingredients, which can solve the problems of single extraction, high extraction cost, low resource utilization rate, etc., and achieve the effects of avoiding resource waste, reducing cost and improving resource utilization rate.

Inactive Publication Date: 2012-09-19
HUBEI CHANGYOU MODERN AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are patent applications for the preparation methods of volatile oil, total flavonoids and total polysaccharides of Ruoye leaves with application numbers 03142883.5, 01111117.8, etc., but these methods are all single extraction, resource utilization rate is low, and extraction cost is high, so it is not suitable for large-scale industrialization. Production

Method used

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  • Method for simultaneously preparing indocalamus leaf volatile oil, total flavone and total polysaccharide
  • Method for simultaneously preparing indocalamus leaf volatile oil, total flavone and total polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 2 kg of Ruoye leaf powder, add 8 times the amount of water, soak for 2 hours, extract by steam distillation for 7 hours, and obtain volatile oil; after extracting the volatile oil, keep the medicinal solution, add 9 times the amount of crude drug to the medicinal residue, and extract 30% ethanol under reflux time, each time 1.0h, filter, after extracting liquid decompression recovery ethanol, combine each extracting liquid and medicinal liquid after extracting volatile oil, decompress and concentrate, obtain extract, add water to disperse and dissolve, make the concentration of loading sample liquid in terms of crude drug amount 0.7g / ml, passed through the AB-8 macroporous resin, the adsorption flow rate was 2BV / h, and the diameter-height ratio of the resin column was 1:6. Precipitate and dry to obtain the total polysaccharide extract of Ruoye leaves; then elute 3BV with 70% ethanol at an elution flow rate of 1.5BV / h, concentrate the eluate and dry to obtain the tot...

Embodiment 2

[0029] Take 5 kg of Ruoye leaf powder, add 5 times the amount of water, soak for 4 hours, extract by steam distillation for 12 hours, and obtain the volatile oil; after extracting the volatile oil, keep the medicinal liquid for use, add the dregs to 12 times the amount of the crude drug, and extract with 40% ethanol under reflux for 3 time, each time 1.5h, filter, after extracting liquid decompression recovery ethanol, combine each extracting liquid and medicinal liquid after extracting volatile oil, decompress and concentrate, obtain extract, add water to disperse and dissolve, make the concentration of loading sample liquid in terms of crude drug amount It is 1.0g / ml, through HP-20 macroporous resin, the adsorption flow rate is 3BV / h, and the resin column diameter-to-height ratio is 1:8. Precipitate and dry to obtain the total polysaccharide extract of Ruoye leaves; then elute 4BV with 70% ethanol at an elution flow rate of 2BV / h, concentrate the eluate and dry to obtain the ...

Embodiment 3

[0031] Take 2kg of Ruoye leaf powder, add 12 times the amount of water, soak for 0h, extract by steam distillation for 4h, and obtain the volatile oil; after extracting the volatile oil, keep the medicinal liquid for use, add the dregs to 6 times the amount of the crude drug, and extract 30% by reflux with 40% ethanol time, each time 1.0h, filter, after extracting liquid decompression recovery ethanol, combine each extracting liquid and medicinal liquid after extracting volatile oil, decompress and concentrate, obtain extract, add water to disperse and dissolve, make the concentration of loading sample liquid in terms of crude drug amount 0.8g / ml, passed through D-101 macroporous resin, the adsorption flow rate was 2BV / h, and the resin column diameter-to-height ratio was 1:7. Precipitate and dry to obtain the total polysaccharide extract of Ruoye leaves; then elute 3BV with 70% ethanol at an elution flow rate of 1BV / h, concentrate the eluate and dry to obtain the total flavonoi...

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Abstract

The invention discloses a method for simultaneously preparing indocalamus leaf volatile oil, total flavone and total polysaccharide, which comprises the following steps: taking indocalamus leaf powder and adding water of 5-12 times in weight; dipping for 0-4h; extracting for 2-12h through a steam distillation method; extracting through organic solvent to obtain the volatile oil; keeping the extracted medicine liquid for later use; adding 20-50% of ethanol of 6-12 times in raw dose mass by weight into the medicine dreg; carrying out backflow extraction for 2-3 times of 0.5-1.5h each time; filtering; combining each extracted liquid and extracting the medicine liquid after extracting the volatile oil; depressurizing and concentrating so as to obtain the extractive; adding water for dissolution; making the concentration of the loading solution be 0.4-1.0 g / ml by raw dose mass; passing through macroporous resin, wherein the absorbing flow speed is 1-3 BV / h, and the resin column height ratio is 1:5-8; firstly eluting using water for 2-4 BV; depositing the eluting liquid using the ethanol; drying to obtain the polysaccharide; subsequently eluting using 40-90% of ethanol for 3-6 BV, wherein the elution flow speed is 0.8-2 BV / h; and concentrating the eluting liquid and drying to obtain the total flavone.

Description

technical field [0001] The invention relates to the technical field of deep processing of agricultural products, in particular to a method for simultaneously extracting volatile oil, flavonoids, polysaccharides and other biologically active ingredients from Ruo leaves. The method involves integrating steam distillation, macroporous resin technology, vacuum concentration technology and other methods for extracting biologically active ingredients from the leaves. Background technique [0002] Ruoye is the leaf of the plant of the genus Indocal.mus in the Graminales subfamily Bambusoideae Nees. It is mainly distributed in the provinces and autonomous regions south of the Yangtze River. Ruo leaves can be used to wrap glutinous rice and cook it into food. This food is one of the traditional delicacies of the Chinese nation. It has a long history in southern my country. It has a special fragrance, is convenient to eat, and is resistant to storage. Modern medicine shows that Ruo ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899C11B9/02A61P1/04A61P29/00A61P35/00A61K127/00
Inventor 陈新张长友易继斌高跃生谭遵伟
Owner HUBEI CHANGYOU MODERN AGRI CO LTD
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