Noninvasive test kit of susceptibility gene of lung squamous carcinoma

Abstract

The invention provides a noninvasive test kit of a susceptibility gene of lung squamous carcinoma. The kit comprises a specific primer of two single nucleotide polymorphism locus gene types for detecting whether Lys75Gln and GSTT1 genes on an XPD (Xeroderma Pigmentosum group D) gene are lacked or not (Present / Null), a DNA (Deoxyribonucleic Acid) sequencing primer, a PCR reaction assembly, a PCR product purifying assembly, a DNA sequencing reaction assembly and the like. The kit is used for estimating a risk grade for a subject suffering the lung squamous carcinoma through detecting the gene types of the two single nucleotide polymorphism loca which are relative to the lung squamous carcinoma; and the subject is guided to prevent the happening of the lung squamous carcinoma in a targeted manner from a gene level according to a gene detection result of each subject, so as to reduce the morbidity of the subject. A sampling method of the invention is as follows: an oral mucosa cell is sampled, the purposes of being painless and noninvasive and avoiding crossed infection can be realized. A sequencing detection result is accurate and reliable, a special imported instrument with expensive cost does not need to be purchased, and the method is easy to popularize and spread.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and specifically relates to a detection kit for susceptibility genes of lung squamous cell carcinoma. Cancer direction guide. Background technique [0002] Squamous cell carcinoma of the lung, also known as squamous cell carcinoma, including spindle cell carcinoma, is the most common type, accounting for about 40%-50% of primary lung cancers. Squamous cell carcinoma of the lung is more common in elderly men and is closely related to smoking. Treatment of squamous cell carcinoma of the lung Most patients are diagnosed with advanced lung cancer. Cough, coughing and chest pain, weight loss and anemia are the main clinical manifestations of small cell lung cancer. The disease is a polygenic disease. Studies have found that the XPD gene and GSTT1 gene play a role that cannot be ignored in the process of lung squamous cell carcinoma, which provides a scientific ...

AI Technical Summary

Problems solved by technology

When Lys→Gln at position 751 of the XPD gene, the ability to repair DNA damage is reduced, the stability of the genome is reduced, and the ability to resist the damage of carcinogens or other external factors is reduced, which increases the risk of cancer

[0004] Molecular biology studies have shown that individuals carrying GSTT1 deletions have lost the entire gene and lost their functions, which is equivalent to increasing the concentration of environmental toxins and carcinogens, increasing the risk of individuals suffering from a series of types of cancer

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