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Hypocrellin high-yielding Shiraia bambusicola strain

A kind of technology of rhodopsin and bamboo yellow fungus, applied in the field of bioengineering, can solve the problems of unfavorable industrialized production, low bacterial yield and the like

Inactive Publication Date: 2013-08-21
苏州圣敏天立生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, the yield of the existing strains is still low, which is not conducive to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The fermented strain is Bamboo yellow fungus ( Shiraia bambusicola WW67), using liquid fermentation, the medium group is glucose 2%, yeast extract 0.5%, MgSO 4 7H2O 0.05%, K 2 HPO 4 0.05%, pH 6.0, inoculum size 10%, fermentation temperature 28°C, 250ml Erlenmeyer bottle liquid volume 20%. After 3 days of fermentation, the content of Hypocretin A, Hypocretin B, Scaboclavin A, Scabcyscin B, Scabcyscin C, and Scaboclavin D in the fermented liquid They are: 0.22%, 0.032%, 0.02%, 0.014%, 0.12%, 0.021%.

Embodiment 2

[0033] The fermented strain is Bamboo yellow fungus ( Shiraia bambusicola WW67), using liquid fermentation, the medium group is fructose 2%, peptone 0.5%, MgSO 4 7H2O 0.05%, KH 2 PO 4 0.05%, pH 7.0, inoculum size 10%, fermentation temperature 30°C, 250ml Erlenmeyer flask liquid volume 20%. After 3 days of fermentation, the content of Hypocretin A, Hypocretin B, Scaboclavin A, Scabcyscin B, Scabcyscin C, and Scaboclavin D in the fermented liquid They are: 0.38%, 0.02%, 0.015%, 0.01%, 0.11%, 0.03%.

Embodiment 3

[0035] The fermentation strain was bamboo yellow fungus ( Shiraia bambusicola WW67), using liquid fermentation, medium group is 3% sucrose, 0.5% beef extract, MgSO 4 7H2O 0.03%, KH 2 PO 4 0.04%, pH 5.0, inoculum size 10%, fermentation temperature 26°C, 250ml Erlenmeyer flask liquid volume 20%. After 3 days of fermentation, the content of Hypocretin A, Hypocretin B, Scaboclavin A, Scabcyscin B, Scabcyscin C, and Scaboclavin D in the fermented liquid They are: 0.22%, 0.01%, 0.014%, 0.02%, 0.10%, 0.023%.

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Abstract

The invention discloses a hypocrellin high-yielding Shiraia bambusicola strain, which has the classified name of Shiraia bambusicola WW67, and was preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO.2012127 on April 22. The strain has the nucleotide sequence shown in SEQ ID NO:1. The main metabolites of the strain are hypocrellin A, hypocrellin B, elsinochrome A, elsinochrome B, elsinochrome C and elsinochrome D.

Description

Technical field [0001] The invention involves a bamboo and molten strain with high -yielding bamboo and cinomin, which belongs to the field of biological engineering technology. Background technique [0002] Bamboo yellow ( Shiraia bambusicola ) Also known as bamboo flowers, bamboo red dumplings, bamboo three seven, bamboo cocoons, etc., is a kind of fungi that is particularly parasites on some bamboo and tender branches. The medicinal is a piece of a piece of a piece of folk medicinal material.The effects of Shujin, removing wind and dampness, nourishing qi, promoting blood circulation and nourishing blood, and dispersing blood stasis can be used to treat deficiency and cold stomach pain, rheumatoid arthritis, bronchitis, pertussis, sciatica pain, bruises and anemia headacheWaiting for the disease.Basiliculin is the most important biological activity ingredient in bamboo and yellow. The physiological functions of bamboo and cibain are mainly: antibacterial and anti -inflammatory...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 蔡宇杰金大勇曹军崔佳龙
Owner 苏州圣敏天立生物科技有限公司
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