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Process for producing salinomycin by glucose supplement and fermentation based on metabolic parameter OUR (oxygen uptake rate)

A technology of salinomycin and sugar supplementation, which is applied in the field of salinomycin production based on metabolic parameter OUR sugar supplementation fermentation process, can solve the problems of increasing the cost of salinomycin fermentation, and achieve the weakening of glucose effect and the improvement of product titer. , the effect of reducing production costs

Active Publication Date: 2012-10-03
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production of salinomycin is mainly carried out by biological fermentation, mainly using soybean oil as its main fermentation carbon source, such as disclosed in Chinese patent CN1230596A, but as the price of oil continues to rise in recent years, the fermentation of salinomycin The cost has been greatly increased accordingly

Method used

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  • Process for producing salinomycin by glucose supplement and fermentation based on metabolic parameter OUR (oxygen uptake rate)
  • Process for producing salinomycin by glucose supplement and fermentation based on metabolic parameter OUR (oxygen uptake rate)
  • Process for producing salinomycin by glucose supplement and fermentation based on metabolic parameter OUR (oxygen uptake rate)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Use media component 1

[0043] (1) Slant culture: Inoculate the Streptomyces albicans strain on a fresh slant and culture at 26°C for 8 days;

[0044] (2) Seed cultivation: insert the spores obtained in step (1) into the seed medium, and cultivate for 30 hours at 30°C and 260 r / min;

[0045] (3) Fermentation culture: Inoculate the seed solution obtained in step (2) into a fermenter (15L tank, about 8 liters of seed medium after sterilization), the inoculation amount is 10%, the culture temperature is 30-35°C, the pressure is 0.05MPa, DO Control it above 30%, and the speed is 250-500rpm. When OUR tends to be stable, start to add glucose with a mass percentage concentration of 60% at a flow rate of 10ml / h. After 280 hours of fermentation, it was put into a tank, and the potency was 72457u / ml, as shown in Table 1.

[0046] Such as figure 1As shown, 0-65h is the sugar-oil conversion period, and OUR rises; 65-110h, the sugar-oil conversion period ends, and OUR begins to r...

Embodiment 2

[0049] Use media component 1

[0050] (1) Slant culture: Inoculate the Streptomyces albicans strain on a fresh slant and culture at 30°C for 6 days;

[0051] (2) Seed cultivation: insert the spores obtained in step (1) into the seed medium, and cultivate for 30 hours at 35°C and 220 r / min;

[0052] (3) Fermentation culture: inoculate the seed liquid obtained in step (2) into a fermenter (30L tank, about 16 liters of seed medium after sterilization), the inoculum amount is 10%, the culture temperature is 30-35°C, the pressure is 0.05MPa, DO Control it above 30%, and the speed is 250-500rpm. When OUR tends to be stable, start to add sucrose with a mass percentage concentration of 40% at a flow rate of 25ml / h. After 300 hours of fermentation, put it into the tank. Titer 65530u / ml.

Embodiment 3

[0054] Use media component 2

[0055] (1) Slant culture: Inoculate the Streptomyces albicans strain on a fresh slant and culture at 28°C for 7 days;

[0056] (2) Seed cultivation: insert the spores obtained in step (1) into the seed medium, and cultivate for 30 hours at 32°C and 240 r / min;

[0057] (3) Fermentation culture: inoculate the seed solution obtained in step (2) into a fermenter (45L tank, about 24 liters of seed medium after sterilization), the inoculum amount is 10%, the culture temperature is 30-35°C, the pressure is 0.05MPa, DO Control it above 30%, and the speed is 250-500rpm. When OUR tends to be stable, start to add glucose with a mass percentage concentration of 50% at a flow rate of 30ml / h. Put the tank after 320 hours of fermentation. Titer 69875u / m.

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Abstract

The invention discloses an optimization method of a fermentation process of salinomycin, and particularly relates to a process for producing salinomycin by glucose supplement and fermentation based on a metabolic parameter OUR (oxygen uptake rate). The fluid glucose supplement is processed when fermentation enters a thalli metabolism stationary phase, namely OUR stationary phase after sugar oil conversion is finished so as to reduce the 'glucose effect', so the valence of the product is improved, and the production cost is reduced at the same time. The process disclosed by the invention has a mature technology and is suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to a method for optimizing the fermentation process of salinomycin, in particular to a process for producing salinomycin by fermenting sugar supplementation based on the metabolic parameter OUR. Background technique [0002] Salinomycin is a polyether monocarboxylic acid antibiotic with a special ring structure and is a typical ionophore antibiotic. Salinomycin is a polyether ionophore-type animal-specific antibiotic produced by Streptomysis albus. It has been recognized by the majority of breeders due to its broad-spectrum and high-efficiency anti-coccidiosis performance. [0003] At present, the production of salinomycin is mainly carried out by biological fermentation, mainly using soybean oil as its main fermentation carbon source, such as disclosed in Chinese patent CN1230596A, but as the price of oil continues to rise in recent years, the fermentation of salinomycin The cost has been greatly improved accordingly. [0004] ...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/47
Inventor 储消和沈建杨小一高圣君蔡群芳黄会鸽
Owner 内蒙古拜克生物有限公司
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