6 results about "Oxygen uptake rate" patented technology

The present invention relates to transcriptional regulator polypeptides, polynucleotides encoding the transcriptional regulator polypeptides, and to nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing a polypeptide of interest in host cells overexpressing the transcriptional regulators, to methods of increasing the oxygen uptake rate and / or decreasing viscosity of the culture broth in a fungal host cell cultivation process, to methods of producing a transcriptional regulator polypeptide, to the use of a transcriptional regulator polypeptide, and to the production of fungal biomass.

The invention belongs to the technical field of sewage treatment, and relates to an activated sludge system gas supply flow control method and application. The specific technical scheme is as follows: (1) in the operation process of the activated sludge sewage treatment system, acquiring the oxygen uptake rate OUR, the dissolved oxygen DO and the temperature T of the activated sludge in an aerobic tank in real time, recording the air supply flow Q, and constructing a training test data set; (2) training an artificial neural network model by taking the oxygen uptake rate OUR, the dissolved oxygen DO and the temperature T as model input and taking the air supply flow Q as model output; and (3) during the operation of the activated sludge sewage treatment system, calculating the corresponding air supply flow Q by using the trained artificial neural network model, and adjusting the actual air supply flow of the aerobic tank of the activated sludge sewage treatment system to the air supply flow Q calculated by the artificial neural network model. The air supply flow is regulated and controlled based on the activated sludge process state, and blindness and hysteresis of air supply flow regulation completely depending on dissolved oxygen (DO) feedback are avoided to a certain extent.

A high-efficiency gas-liquid dispersion stirrer comprises a disc and a plurality of blades uniformly distributed on the periphery of the disc, the blades comprise upper blades and lower blades which are arranged in a split mode, the upper blades and the lower blades form a V-shaped dispersion groove which is consistent with the rotation direction of the disc, and the diameters of the two ends of the dispersion groove are gradually increased from inside to outside. A small opening with a gap is formed in one side, with a smaller caliber, of the dispersion groove, and a large opening with a larger caliber is formed in the other side of the dispersion groove. When the stirrer runs and compressed air passes through a rotation area of the stirrer, the compressed air is sucked into air holes generated when the backs of the V-shaped blades run, and when the air holes are exploded under the action of centrifugal force, the air holes in the backs of the blades become smaller due to inflow of liquid in gaps at small openings of the blades, so that the blades are driven to rotate. Therefore, the cavitation can be more favorably divided into a plurality of fine bubbles, the contact surface area of the bubbles and liquid is greatly increased, and the gas-liquid dispersing capacity of the stirrer and the dissolved oxygen rate of a medium are more effectively improved.

The invention provides a dynamic carbon accounting method for biological fermentation of vitamins and application of the dynamic carbon accounting method, and belongs to the technical field of carbon emission control. Comprising the following steps: constructing a dynamic carbon balance equation of biological fermentation vitamins to track input, conversion and output of carbon elements in a fermentation tank in real time; establishing a detection sensor matrix to collect process data corresponding to each parameter in the equation in real time; a long-short-term memory network model is trained based on historical fermentation batch data, input quantities comprise dissolved oxygen, pH, oxygen uptake rate and feeding rate which are collected in real time, and output is real-time carbon conversion efficiency; in the fermentation process, various parameters in the equation are calculated by using process data collected by a detection sensor matrix, and the calculated value of the fixed carbon amount of riboflavin carbon synthesis in unit time is dynamically corrected by using the real-time carbon conversion efficiency output after model training, so that the corrected riboflavin carbon generation rate is obtained. According to the invention, the dynamic correction of the carbon conversion efficiency is realized, and the real-time performance and precision of carbon accounting are improved.

The invention relates to the technical field of sewage treatment automation control, in particular to a sewage treatment equipment parameter tracking control method. The method comprises the steps of data acquisition, load prediction, state evaluation, prediction control and feedback correction. The system determines a biochemical operation state by acquiring water inlet parameters and real-time data of a reaction area; the core of the method is to construct a virtual water mass tracking model to generate a future load impact curve, evaluate biological reaction inertia based on an oxygen uptake rate, and output actual oxygen demand and metabolic response delay time; after the impact arrival time is aligned with the metabolic delay, generating an elastic tracking envelope by using a model predictive control algorithm, adaptively adjusting the aeration equipment, and performing closed-loop correction based on the effluent quality; according to the method, conversion from passive hysteresis control to accurate feedforward prediction is realized, inflow disturbance and metabolic inertia can be coordinated in advance, and insufficient oxygen supply and excessive aeration are effectively avoided.

This invention provides a fermentation method for N-acetylglucosamine based on oxygen uptake rate regulation, comprising the following steps: S1: Activating and seeding an engineered bacterium that produces N-acetylglucosamine through induction with isopropyl-β-D-thiogalactoside to obtain a seed culture; S2: Inoculating the seed culture obtained in step S1 into a fermenter containing fermentation medium for fermentation, monitoring the oxygen uptake rate (OUR) of the fermentation broth during fermentation, and adding ammonium sulfate when the OUR rises; S3: Adding glucose solution during fermentation and continuing cultivation until the fermentation endpoint. This method, by monitoring the oxygen uptake rate in real time during fermentation and using its characteristic rise as the trigger signal for adding ammonium sulfate, achieves precise intervention at the metabolic inflection point of the bacteria, effectively inhibiting the abnormal rise of OUR caused by metabolic imbalance, prolonging the efficient synthesis period of the product, and thus significantly improving the yield and conversion rate of N-acetylglucosamine.