Sensing chip of dual-detecting biochemical sensing detector and preparation method thereof

Abstract

The invention discloses a sensing chip of a dual-detecting biochemical sensing detector and a preparation method thereof. The sensing chip comprises a sensing chip unit arranged in a K*L array, wherein the sensing chip unit comprises a substrate and an upper slice bonded with the substrate together; an inlet, an outlet and a microcavity which are formed in the upper slice to form a micro-flow channel; and nano particles arranged in an m*n array, which are formed on the substrate and located in the microcavity, wherein m, n, K and L are natural number. The sensing chip provided by the invention is a multi-unit and multi-array structure and combined with a micro-flow control technology to integrate a micro-flow control optical sensing system. With the adoption of a nano processing technique with low cost, the sensing chip can be processed on a large area; furthermore, the sensing chip is good in repeatability, simple in preparation method and few in required samples; the sensing chip can observe in real time, perform the parallel test of multiple channels and parameters and the tests of in-situ surface enhanced raman scattering (SERS) and localized surface plasmon resonance (LSPR).

Description

technical field [0001] The invention belongs to the field of biochemical sensing and detection, and relates to a sensor chip of a surface-enhanced Raman spectrum integrated microfluidic optics-localized surface plasmon resonance dual-detection biochemical sensing detector and a preparation method thereof. Background technique [0002] A large number of studies have shown that the optical properties of metal nanoparticles are the localized surface plasmon resonance (LSPR) frequency, which is closely related to the composition, spacing, size, shape and local dielectric environment of metal nanoparticles. Therefore, as a potential spectral analysis technology based on localized surface plasmon sensor chips, it has been widely used in various aspects such as physics, chemistry, medicine, environmental monitoring, and public safety. The shape, size, and spacing of metal nanostructures have an impact on the optical properties of LSPR and the SERS enhancement effect of surface-enha...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the deficiencies in the traditional single testing technology LSPR or SERS technology, using local surface plasmon resonance LSRP technology, microfluidic technology and micro-nano processing technology to realize a high-sensitivity, label-free nano-array biochemical detector, the present invention proposes A Novel Metal Nanostructure—Sensing Chip for Dual-Detection Biochemical Sensing Detector

Images