Tissue culture container and tissue culture method

Abstract

The invention discloses a tissue culture container and a tissue culture method. The tissue culture container comprises a culture container body with a bottom wall and at least one side wall to limit an inner cavity, the culture container body comprises a cover, the inner cavity internally comprises at least one bowl-shaped container, each bowl-shaped container comprises a hemispheric bowl bottom and a mouth, a flange perpendicular to the side wall of each bowl-shaped container is arranged at the position of each mouth, the diameter of the inner edge of each flange is smaller than that of each mouth, and the diameter of the outer edge of each flange is larger than that of each mouth, and the bottom of each bowl-shaped container is connected with the bottom of the culture container body. The tissue culture method includes the steps: (1) enveloping each bowl-shaped container by hydrophilic gelatin; (2) adding culture solution; (3) cutting and adding tissue blocks; and (4) inversely placing the culture container body into an incubator. A gas-liquid coexisting culture environment required by tissue culture is created by arranging the bowl-shaped containers in the inner cavity of a common culture container, and the tissue culture container is convenient to use, low in cost, fine in effect and particularly suitable for culture of tissue blocks small in size and large in density.

Description

technical field [0001] The invention relates to a culture vessel specially used for tissue culture and a tissue culture method using the culture vessel. Background technique [0002] Tissue engineering is a frontier subject in current life science research, and human health cannot be separated from the development and improvement of tissue engineering science. Traditional body repair mostly uses autologous tissue transplantation. Although it can achieve satisfactory results, it is a conservative method at the expense of autologous healthy tissue, which will eventually lead to many complications and additional secondary damage. On top of that, some important tissues in the human body are unique within the body, for example, the cornea. After the cornea is damaged, there is no replacement tissue in the body, only other donors or artificial ones can be used. However, at present, the sources of donor corneas donated by human beings are very limited, and the health recovery of ...

AI Technical Summary

Problems solved by technology

However, the current structure of conventional cell culture vessels cannot provide an ideal gas-liquid coexistence culture environment and cannot meet the needs of tissue culture.

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