Chemiluminescence immunodetection kit and detection method for ovarian cancer tumor marker HE4

A chemiluminescent immune and tumor marker technology, applied in the field of biological and medical detection, can solve the problems of non-detection and achieve good linear range, high affinity, curative effect observation and reliable prognosis judgment

Inactive Publication Date: 2012-10-17
河南生生医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] CA125 is one of the most sensitive indicators for diagnosing ovarian cancer and monitoring its recurrence, but CA125 is often not detected in the early stages of disease, and its clinical application has certain limitations

Method used

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  • Chemiluminescence immunodetection kit and detection method for ovarian cancer tumor marker HE4
  • Chemiluminescence immunodetection kit and detection method for ovarian cancer tumor marker HE4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The ovarian cancer tumor marker HE4 chemiluminescent immunoassay kit provided in this example includes a 9F3 antibody-coated microwell plate, an alkaline phosphatase-labeled 10E1 antibody, an analysis buffer, a substrate working solution, a luminescent substrate, Washing solution, quality control products.

[0031] The preparation method of the 9F3 antibody-coated microwell plate is as follows: dilute the 9F3 antibody to 0.1 μg / μl with coating buffer to prepare the 9F3 antibody coating solution, and then add 50 μg / μl to each well of the microwell plate. Microliter of 9F3 antibody coating solution was coated at 37°C for 2 hours, then the microplate was rinsed three times with normal saline, and then 100 microliter of sealing solution was added to each well of the microplate, and kept at room temperature After blocking for 2 hours, the microwell plate was rinsed twice with normal saline, and freeze-dried to prepare a 9F3 antibody-coated microwell plate, which was sealed i...

Embodiment 2

[0053] The chemiluminescent immunoassay method for the ovarian cancer tumor marker HE4 provided in this example adopts the kit provided in Example 1 of the present invention, and includes the following steps:

[0054] (1) Add BSA to the PBS buffer to prepare a PBS buffer with a BSA mass percentage concentration of 1% BSA, dilute the purchased recombinant HE4 protein with this buffer, and prepare a recombinant HE4 protein standard with a series of concentration gradients solution;

[0055] (2) Take 50 microliters of the human serum to be tested and 50 microliters of the recombinant HE4 protein standard solution with a series of concentration gradients, add them to different wells of the 9F3 antibody-coated microwell plate, and then add the ALP-labeled 10E1 antibody Working solution (1:2000) 50 microliters, then add luminescent substrate CSPD, shake evenly, incubate at 37°C for 1 hour, wash 5 times with washing solution, dry on absorbent paper, then add substrate working solutio...

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Abstract

The invention relates to the field of biological and medical detection technologies, specifically discloses a chemiluminescence immunodetection kit for the ovarian cancer tumor marker HE4 and also discloses a method of using the chemiluminescence immunodetection kit for the ovarian cancer tumor marker HE4 for detecting the ovarian cancer tumor marker HE4. The chemiluminescence immunodetection kit comprises a microplate coated by 9F3 antibodies, 10 E1 antibodies labeled by alkaline phosphatase, an analysis buffer solution, substrate operating fluid, a luminescent substrate, washing liquid and a quality control. The chemiluminescence immunodetection kit for the ovarian cancer tumor marker HE4 provided in the invention has a linear range of 0.5 ng/ml to 1200 ng/ml and a detection limit of 0.2 ng/ml, can be used for clinical auxiliary diagnosis, curative effect observation and prognosis of ovarian cancers and has important significance to treatment and prevention of ovarian cancer tumors.

Description

technical field [0001] The present invention relates to the technical field of biological and medical detection, in particular to an ovarian cancer tumor marker HE4 chemiluminescence immunoassay kit, and also relates to the detection of ovarian cancer tumor markers by using the ovarian cancer tumor marker HE4 chemiluminescence immunoassay kit HE4 method. Background technique [0002] Ovarian cancer has an insidious onset and poor prognosis, and is described as one of the deadliest cancers. The early symptoms of ovarian cancer are not obvious. The initial symptoms are abdominal pain or distension, and indigestion of the stomach. It is not easy to attract enough attention. Generally, ovarian cancer patients are discovered at an advanced stage, so the cure rate is extremely low. If ovarian cancer can be found in the early stage, the cure rate of ovarian cancer can be greatly improved. In order to achieve this goal, people have done a lot of research work. [0003] CA125 is on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/574
Inventor 王嘎程自卿
Owner 河南生生医疗器械有限公司
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