Manufacture method of corncob halimasch production seeds

Abstract

The invention relates to a method for manufacturing halimasch production seeds from corncob grains. The method comprises the following steps of (1) material proportioning: corncob grains, wheat bran, corn powder and gypsum are taken according to weight parts; (2) material stirring: all ingredients are mixed and uniformly stirred, then, water is added until the water content in culture materials reaches 60 percent to 80 percent, and the materials are uniformly stirred; (3) bag loading: the uniformly stirred culture materials are loaded into material bags made of polyethylene or polypropylene materials; (4) sterilization: the material bags are sterilized at high pressure or normal pressure; (5) inoculation: halimasch strain blocks are dug and taken from an original strain bottle and are transferred into the material bags; and (6) culture: the inoculated material bags are transferred into a lucifugal culture room to be cultured under the temperature condition being 22 to 25 DEG C until the halimasch rhizomorph grows all over the material bags. The method provided by the invention has the advantages that the production cost is reduced, the finished product rate of the product is improved, and the great consumption of wood materials is avoided, so forest and the ecological environment are protected. The halimasch production seeds obtained by adopting the method can be used for bacterium material culture in the gastrodia elata planting.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to the preparation of a production species of Armillaria corncob. Background technique [0002] Gastrodia elata (Gastrodia elata): a traditional precious Chinese medicinal material in my country. It is a perennial herb of Orchidaceae. It has no roots and leaves. Like ordinary green plants, it cannot absorb nutrients in the soil through roots, nor can it synthesize the substances it needs through photosynthesis. , in the process of its growth, it is necessary to provide nutrition for it through a fungus - Armillaria mellea. [0003] Armillaria mellea (Armillaria mellea): a large fungus that can produce fruiting bodies (mushrooms). It is a wood saprophytic fungus that often grows on dead wood or stakes. Mycelia and hyphae can be combined with Gastrodia elata tubers to provide Gastrodia elata with the nutrients needed for growth. [0004] Mycelium: A root-like tissue composed...

AI Technical Summary

Problems solved by technology

There are following disadvantages in the existing production technology of Armillaria armillaria: (1) due to the large-scale production of strains requires a large amount of miscellaneous wood materials, which will cause certain damage to forest and ecological environment; (2) Because of the sharp edges and corners of the sticks, plastic fungus bags cannot be used as containers, and cans, glass or polyethylene (propylene) special strain bottles must be used as containers, and the cost of canned bottles or strain bottles is too high; (3) the The technology needs to cut and cut materials, and the bottling process needs to be done manually instead of mechanized operation, and the labor cost is high; (4) The material needs to be filled with water after bottling, and the material bottle is easily overturned due to air flow during the sterilization process, and The water in the bottle is easy to expand and overflow under high temperature conditions, and it is easy to break through the sealing paper, which affects the success rate of bottle making. In addition, after the water overflows, the organic matter adheres to the outer wall of the bottle mouth, and the contamination rate of bacteria is high in the later cultivation process; (5) The amount of bacteria in a single bottle of bottled bacteria is small, and the mushroom stick is used as an independent inoculation unit, the inoculation point is small, and the amount of bacteria used per square meter is relatively large. Conventionally, 4-6 bottles are required for culture of bacteria per square meter Bacteria, the market price of each bottle of bacteria is about 2.5 yuan, therefore, it needs 10-15 yuan of bacteria per square meter, the price is too high