Method for extracting and separating cordycepin

A technology for cordycepin and extraction solution, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of difficulty in sample processing, low extraction rate of cordycepin, and low purity.

Inactive Publication Date: 2012-10-24
JILIN QITAI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low extraction rate and low purity of cordycepin due to the easy heat generation in the existing cordycep...

Method used

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  • Method for extracting and separating cordycepin
  • Method for extracting and separating cordycepin
  • Method for extracting and separating cordycepin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054]The raw material of Cordyceps sinensis fruiting body is pulverized by a low-temperature pulverizer (Shanghai Like Polymer Materials Co., Ltd.) with liquid nitrogen as a medium at -196 ° C, and passed through a 200-mesh sieve to obtain powder; the obtained powder and distilled water are in a weight ratio 1:5 mixing, ultrasonic extraction was carried out under the condition of ultrasonic frequency (Japan Honda W-115 ultrasonic cleaning machine) 28KHz, output power 200W, extraction time was 30min, and then centrifuged at 5000 rpm / centrifuge (high-speed refrigerated centrifuge GL -20B) Filtration for 15min to obtain the extract, which was analyzed by liquid chromatography and found that the mass content of cordycepin was 0.0010%; the extract was mixed with macroporous resin (produced by Tianjin Haiguang Chemical Co., Ltd., model NKA II), and the temperature was 20°C. , the loading volume is 10 times the resin volume, the adsorption flow rate is 3 times the resin BV / hr, the re...

Embodiment 2

[0056] The raw material of Cordyceps sinensis mycelium was pulverized by a low temperature pulverizer (Shanghai Like Polymer Materials Co., Ltd.) with liquid nitrogen as a medium at -196 °C, and passed through a 700-mesh sieve to obtain powder; 1:10 mixing, ultrasonic extraction was carried out under the condition of ultrasonic frequency (Japan Honda W-115 ultrasonic cleaning machine) 30KHz, output power 100W, extraction time was 20min, and then centrifuged at 5000 rpm / centrifuge (high-speed refrigerated centrifuge GL -20B) Filtration for 15min to obtain the extract, analyzed by liquid chromatography, the mass content of cordycepin is 0.0012%, such as figure 1 (the peak marked with * in the figure represents cordycepin); the extract was mixed with macroporous resin (produced by Tianjin Haiguang Chemical Co., Ltd., model NKAⅡ), the temperature was 20°C, and the loading volume was 10 times the resin volume. The adsorption flow rate was 1 times the resin BV / hr, and the resin colu...

Embodiment 3

[0058] The raw material of the fruiting body of Cordyceps sinensis is pulverized by a low temperature pulverizer (Shanghai Like Polymer Materials Co., Ltd.) with liquid nitrogen as a medium at -196 ° C, and passed through a 500-mesh sieve to obtain a powder; the obtained powder and distilled water are in a weight ratio of 1 :20 mixing, ultrasonic extraction was carried out under the condition of ultrasonic frequency (Japan Honda W-115 ultrasonic cleaning machine) 20KHz, output power 100W, extraction time was 10min, and then centrifuged at 5000 rpm / centrifuge (high-speed refrigerated centrifuge GL- 20B) Filtration for 15 minutes to obtain the extract, which was analyzed by liquid chromatography and found that the mass content of cordycepin was 0.0011%; the extract was mixed with macroporous resin (produced by Tianjin Haiguang Chemical Co., Ltd., model NKA II), and the temperature was 20°C. The loading volume is 10 times the resin volume, the adsorption flow rate is 1.5 times the...

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Abstract

The invention provides a method for extracting and separating cordycepin, belonging to the technical field of intensive process of agricultural products and aiming to solve the problems caused by easily-generated heat in existing cordycepin method that extraction rate and purity are low, only dry samples can be processed while high water content samples are difficult to process. The method comprises the steps of crushing snowflake cordyceps sinensis at low temperature; mixing the obtained material with water in a ratio of 1:5-1:20; after ultrasonic extraction under the condition of 20-30 kHz ultrasonic frequency, mixing and balancing in macroporous resin; purifying by using reverse phase high performance liquid chromatography; and finally obtaining the cordycepin concentrate through desalination using nanofiltration and drying. The method employs low-temperature liquid nitrogen for grinding, combining with low power low-frequency ultrasonic output to promote dissolution, so the mass content of the cordycepin can reach 98-99% at most and the recovery rate can reach 88-90%.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, and particularly relates to a method for extracting and separating cordycepin. Background technique [0002] Cordycepin, also known as cordycepin, cordycepin militaris, and 3'-deoxyadenosine, is the first nucleoside antibiotic isolated from fungi. Its molecular formula is C 10 H 13 N 5 O 3 , molecular weight 25l.24, melting point 230-23l ℃, soluble in water, hot ethanol and methanol, insoluble in benzene, ether and chloroform, the maximum absorption wavelength of ultraviolet light is 259nm. Cordycepin has many pharmacological effects such as anti-tumor, anti-leukemia, antibacterial, immune regulation, and scavenging free radicals in the body. It is also reported that on the one hand, cordycepin has three inhibitory effects on the synthesis of DNA and RNA, the post-transcriptional modification of RNA and mRNA, and the activation of adenylate cyclase. On the ot...

Claims

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Application Information

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IPC IPC(8): C07H19/16C07H1/08
Inventor 王秀然成怀畅太龙杰太青林
Owner JILIN QITAI TECH
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