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Cucumber vein yellowing virus (CVYV) resistant cucumber plants (Cucumis sativus L.)

A yellowing virus and cucumber technology, applied in plant products, plant genetic improvement, botany equipment and methods, etc., can solve the problems of yield reduction and other issues

Inactive Publication Date: 2012-11-07
ENZA ZADEN BEHEER BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rapid spread of virus in greenhouse can lead to significant yield loss

Method used

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  • Cucumber vein yellowing virus (CVYV) resistant cucumber plants (Cucumis sativus L.)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Genetic Components Conferring Resistance to Cucumber Vein Yellow Virus (CVYV)

[0045] illustrate

[0046] In this example, performed by Keygene N.V, Wageningen, The Netherlands, bulk QTL analysis (BulkQTL Analysis, B QA) was used in resistant cucumber plants (Cucumis sativus L.) to identify Quantitative trait locus (Qualitative Trait Locus, QTL) of the genetic component of yellowing virus (CVYV) resistance. Populations were selected for marker development for cucumber vein yellowing virus (CVYV) resistance. A total of 96 AFLP primer combinations were screened across two pools containing individuals displaying an 'extreme phenotype'. Subsequently, five candidate markers were validated on resistant and sensitive samples.

[0047] Mark identification and verification

[0048] The leaf material of the plant was obtained: a population of line 11366x OK561 provided the population for testing, the parental line 11366 of the supplier and the inbred line of th...

Embodiment 2

[0061] Example 2 Molecular markers that aid in the identification of resistant cucumber (Cucumis sativus L.) resistant to cucumber vein yellowing virus (CVYV)

[0062] molecular analysis

[0063] Genomic material was isolated from susceptible and resistant cucumber plants (Cucumis sativus L.), sensitive ie showing one or more symptoms of viral infection, using a standard experimental design.

[0064] Next, the gene material is digested with the appropriate restriction enzymes (EcoRI / Msel) and, after adapter ligation, primed with the primer pair 5-GAC TGC GTA CCA ATTCGT-3′ and 5′-GAT GAG TCC TGA GTA ACA C-3', or primer pair 5'-GAC TGC GTA CCA ATT CAT-3' and 5-GAT GAG TCC TGAGTA ACG T-3', for AFLP nucleic acid amplification. The resulting amplification products were resolved by gel electrophoresis for molecular determination. The presence of the AFLP marker in the genome of individual cucumber plants (Cucumis sativus L.) was detected as absent (-) or present (+).

[0065]Sp...

Embodiment 3

[0070] Example 3 marker 22 / M48-F-246 (which shows the presence of a genetic element conferring resistance to cucumber vein yellowing virus (CVYV)) additionally indicates the presence of a genetic element conferring resistance to cucumber green mottle mosaic virus (CGMMV) and and / or a genetic component conferring resistance to Cucumber Fruit Mottle Mosaic Virus (CFMMV).

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Abstract

The present invention relates to molecular markers genetically linked to, and capable of identifying, a genetic locus in the cucumber plant (Cucumis sativus L.) genome conferring a general resistance against tobamoviruses, and especially against two commercially important pathogenic tobamoviruses, i.e., cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV). The present invention further relates to methods for providing a cucumber plant (Cucumis sativus L.) plants, plant parts and fruits with resistance against tobamoviruses and especially against two commercially important pathogenic tobamoviruses, i.e., cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV).

Description

technical field [0001] The present invention relates to a cucumber vein yellowing virus (CVYV) resistant cucumber plant (CUCUMIS SATIVUS L.), provides a method for cucumber vein yellowing virus (CVYV) resistant cucumber plant (CUCUMIS SATIVUS L.), molecular markers in the method Uses in , and plants, plant parts, tissues, cells and / or seeds derived from or derived from cucumber plant (CUCUMIS SATIVUSL.) resistant to cucumber vein yellow virus (CVYV) of the present invention. Background technique [0002] Cucumber plants, plants of the species Cucumis sativus, are melons that belong to the Cucurbitaceae family, which also includes members of the family like melons and squashes. The edible fruit of this plant is commonly known as cucumber. [0003] Cucumbers are generally cylindrical, green-skinned fruits that comprise approximately 96% water. The cucumber plant, also named Cucumis sativus L., was cultivated very early and is an important horticultural crop in the world. Cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04C12Q1/68A01H5/08
CPCC12Q2600/13C12Q1/6895A01H1/04A01H5/08A01H6/346A01H1/126
Inventor 亚普·马泽利乌布莱特·凡·坎彭罗纳尔多·威尔特丁克南内·法贝尔
Owner ENZA ZADEN BEHEER BV
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