Biological adhesion type cordyceps militaris liposome and preparation method thereof

A technology of liposome and Cordyceps militaris, which is applied in the field of biomedical materials, can solve the problems of low content of active ingredients, difficult to control absorption efficiency, etc., and achieves the effects of uniform distribution, improved drug loading and encapsulation efficiency, and stable properties.

Inactive Publication Date: 2013-11-27
广州安德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although researchers have developed a variety of functional foods using Cordyceps militaris as raw materials, including Cordyceps militaris drinks, health wine, nutritional health vinegar, Cordyceps yogurt, Cordyceps bread, etc., most of them are soaked in its fruiting body or solid medium. The extract is used as a raw material, and it is simply mixed into beverages, wine, and vinegar. The content of active ingredients is very small, and the absorption efficiency is not easy to control

Method used

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  • Biological adhesion type cordyceps militaris liposome and preparation method thereof
  • Biological adhesion type cordyceps militaris liposome and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Precisely weigh 30mg of Cordyceps militaris sporocarp powder and dissolve it in 15mL of water, and take another 100mg of phospholipids, 1.5mg of vitamin C and 1mg of vitamin E, and dissolve them together in 2mL of absolute ethanol; magnetically stir the Cordyceps militaris sporocarp at a speed of 1000r / min At the same time, slowly drop the ethanol solution dissolved in phospholipids, vitamin C and vitamin E into the aqueous solution of the micropowder, and transfer it to the ultrasonic probe for ultrasonication after the addition is completed. After sonication, the sample was transferred to a rotary evaporator, and the ethanol was evaporated at 250 r / min for 1 h at 200 mbar, 40 °C. In addition, 10 mg of hydroxypropyl methylcellulose was weighed, dissolved in 2 mL of water, and the solution was slowly and uniformly dropped into the liposomes, incubated at room temperature for 2 hours, and then placed in a freeze dryer to freeze-dry, and the freeze-dried samples That is C...

Embodiment 2

[0040] Precisely weigh 50 mg of Cordyceps militaris sporocarp powder and dissolve it in 15 mL of water, and take another 150 mg of phospholipids, 2 mg of vitamin C and 1 mg of vitamin E, and dissolve them together in 2 mL of absolute ethanol; magnetically stir the Cordyceps militaris sporocarp powder at a speed of 1000 r / min At the same time, the ethanol solution dissolved in phospholipids, vitamin C and vitamin E was slowly added dropwise. After the dropwise addition, it was transferred to an ultrasonic probe for ultrasonication. After sonication, the sample was transferred to a rotary evaporator, and the ethanol was evaporated at 250 r / min for 1 h at 200 mbar, 40 °C. Another weighed 10 mg of carbomer was dissolved in 2 mL of water, and the solution was slowly and uniformly added dropwise to the liposomes, incubated at room temperature for 2 hours, and then placed in a freeze dryer to freeze-dry, and the freeze-dried sample was Cordyceps militaris Fruiting bodies micronized l...

Embodiment 3

[0043] Precisely weigh 40mg of Cordyceps militaris sporocarp powder and dissolve it in 20mL of water, and take another 100mg of phospholipids, 2mg of vitamin C and 1mg of vitamin E, and dissolve them together in 2mL of absolute ethanol; magnetically stir the Cordyceps militaris sporocarp powder at a speed of 1000r / min At the same time, the ethanol solution dissolved in phospholipids, vitamin C and vitamin E was slowly added dropwise. After the dropwise addition, it was transferred to an ultrasonic probe for ultrasonication. The ultrasonic amplitude was 20%, the ultrasonic time was 5min, and the ultrasonic interval was 5s. After sonication, the sample was transferred to a rotary evaporator, and evaporated at 1500 mbar, 45° C., 250 r / min for 1 h to remove ethanol. Weigh 10 mg of sodium alginate, dissolve it in 2 mL of water, add the solution dropwise slowly and uniformly to the liposomes, incubate at room temperature for 2 hours, and then freeze-dry in a freeze dryer. The freeze-...

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Abstract

The invention discloses a biological adhesion type cordyceps militaris liposome and a preparation method thereof. According to the invention, cordyceps militaris fruiting body micro powder is taken as a raw material, phospholipid is taken as a capsule wall material, surface modification is carried out by virtue of biological adhesion material, and cordyceps militaris fruiting body micro powder liposome is prepared by adopting an ethanol inpouring method. The particle size of the cordyceps militaris fruiting body micro powder liposome prepared by the invention is in nano grade, and the cordyceps militaris fruiting body micro powder liposome has uniform distribution and stable properties. In the obtained liposome, cordyceps militaris polysaccharide is taken as a detection index, the highest detected polysaccharide medicine carrying rate can be 40%, and the highest encapsulated ratio can be about 70%. By adopting the preparation method disclosed by the invention, medicine carrying rate and encapsulated ratio of active ingredients in the liposome can be improved, and automatic and large-scale production can be realized. Surface modification is carried out on the liposome with chitosan, stability of the liposome is enhanced while active ingredients of a cordyceps militaris fruiting body can be better absorbed and utilized, and the active ingredients can be maintained in vivo for a longer time by virtue of slow release effect of the liposome.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a bioadhesive liposome of Cordyceps militaris and a preparation method thereof. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is a complex composed of two parts: the sclerotium (that is, the grass part) and the sclerotia (that is, the dead body part of the insect). Traditional Chinese medicine believes that Cordyceps enters the lung and kidney meridians, which can not only nourish the lung yin, but also nourish the kidney yang. It is the only traditional Chinese medicine that can balance and regulate yin and yang at the same time. [0003] Modern medicine has proved that Cordyceps militaris contains a variety of physiologically active substances, which have antibacterial, anti-cancer, anti-platelet aggregation, anti-radiation, improve and enhance memory, regulate the body's immunity, prevent calcium ion antagonism, and prote...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A23L1/29A23P1/06A23L33/00A23P10/40
Inventor蒋庆于艳君
Owner广州安德生物科技有限公司